Antigen: csA (contact site A glycoprotein)
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Host Species: mouse
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes: Western blot is functional with the fully glycosylated 80kD band. The functional effects of the antigen cross-reacts growth, aggregation and slug phases.
Predicted Species Reactivity:
Immunogen: csA (contact site A glycoprotein)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: PA1
Epitope Location or Sequence:
Antibody Registry ID: AB_1553683
Additional Information: csA from strain Ax2. RRID:AB_1553683
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20-53-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 20-53-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.