Antigen: csA (contact site A glycoprotein)
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Host Species: mouse
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes: Western blot is functional with the fully glycosylated 80kD band. The functional effects of the antigen cross-reacts growth, aggregation and slug phases.
Antigen Molecular Weight: 80kD
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: csA (contact site A glycoprotein)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: PA1
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_1553683
Additional Information: csA from strain Ax2. RRID:AB_1553683
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20-53-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 20-53-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).