20B megalin
Catalog Fields
Clone ID/Product Name: 20B megalin
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Lrp2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2753214
Uniprot ID: P98158
Entrez Gene ID: 29216
Clonality: Monoclonal
Immunogen: Rat glomeruli membrane extract
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63/Ag8.653
Epitope Mapped: No
Antigen Name: Megalin; gp330; Low-density lipoprotein receptor-related protein 2
Epitope Location or Sequence: Ectodomain of megalin
Alternate Antigen Name:
Deposit Date: 2/14/2018
Antigen Molecular Weight: 330 kDa; predicted 519 kDa
Depositor: Farquhar, M.G.
Antigen Sequence:
Depositor Institution: University of California, San Diego
Antigen Species: Rat
Depositor Notes: This antibody recognizes the ectodomain of megalin (gp330) and is useful for endocytic uptake studies.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information:
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20B megalin was deposited to the DSHB by Farquhar, M.G. (DSHB Hybridoma Product 20B megalin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication