20B megalin

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SKU: 20B megalin

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 20B megalin
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Lrp2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2753214 
Uniprot ID: P98158 
RRID:  
Entrez Gene ID: 29216 
Clonality: Monoclonal
Immunogen: Rat glomeruli membrane extract
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63/Ag8.653
Epitope Mapped: No
Antigen Name: Megalin; gp330; Low-density lipoprotein receptor-related protein 2
Epitope Location or Sequence: Ectodomain of megalin
Alternate Antigen Name:
Deposit Date: 2/14/2018
Antigen Molecular Weight: 330 kDa; predicted 519 kDa
Depositor: Farquhar, M.G.
Antigen Sequence:
Depositor Institution: University of California, San Diego
Antigen Species: Rat
Depositor Notes: This antibody recognizes the ectodomain of megalin (gp330) and is useful for endocytic uptake studies.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20B megalin was deposited to the DSHB by Farquhar, M.G. (DSHB Hybridoma Product 20B megalin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibodies against membrane proteins of the rat glomerulus. Immunochemical specificity and immunofluorescence distribution of the antigens.
    Farquhar MG
    The American journal of pathology 137.4 (1990 Oct): 929-44.

    ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression.
    Farquhar MG
    The Journal of cell biology 202.1 (2013 Jul 8): 113-27.

    The adaptor protein ARH escorts megalin to and through endosomes.
    Farquhar MG
    Molecular biology of the cell 14.12 (2003 Dec): 4984-96.

    GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 13.4 (2002 Apr): 918-927.

    Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 11.12 (2000 Dec): 2167-2178.

    IHC References
    IP References
    ELISA References

    Pathogenic antibodies inhibit the binding of apolipoproteins to megalin/gp330 in passive Heymann nephritis.
    Orlando RA
    The Journal of clinical investigation 100.9 (1997 Nov 1): 2303-9.

    FB References
    All References

    Monoclonal antibodies against membrane proteins of the rat glomerulus. Immunochemical specificity and immunofluorescence distribution of the antigens.
    Farquhar MG
    The American journal of pathology 137.4 (1990 Oct): 929-44.

    ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression.
    Farquhar MG
    The Journal of cell biology 202.1 (2013 Jul 8): 113-27.

    The adaptor protein ARH escorts megalin to and through endosomes.
    Farquhar MG
    Molecular biology of the cell 14.12 (2003 Dec): 4984-96.

    GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 13.4 (2002 Apr): 918-927.

    Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 11.12 (2000 Dec): 2167-2178.

    Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells.
    Saito A
    Kidney international 75.12 (2009 Jun): 1308-1315.

    Megalin is an endocytic receptor for insulin.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 9.10 (1998 Oct): 1759-66.

    Role of megalin in endocytosis of advanced glycation end products: implications for a novel protein binding to both megalin and advanced glycation end products.
    Gejyo F
    Journal of the American Society of Nephrology : JASN 14.5 (2003 May): 1123-31.

    The glomerular epithelial cell anti-adhesin podocalyxin associates with the actin cytoskeleton through interactions with ezrin.
    Farquhar MG
    Journal of the American Society of Nephrology : JASN 12.8 (2001 Aug): 1589-1598.

    Pathogenic antibodies inhibit the binding of apolipoproteins to megalin/gp330 in passive Heymann nephritis.
    Orlando RA
    The Journal of clinical investigation 100.9 (1997 Nov 1): 2303-9.

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