21-5E1

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$40.00
SKU: 21-5E1
View product citations for antibody 21-5E1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Nuclear marker, CNS
Hybridoma Cells Available: Yes
Antigen Species: None Known
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Rat
Depositors Notes: This antibody is an excellent IHC marker for nuclei in brain sections but has low affinity for free benzodiazepines.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Benzodiazepine-BSA
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
21-5E1 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 21-5E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    All References

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

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