210-183-1

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$40.00
SKU: 210-183-1
View product citations for antibody 210-183-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Fimbrin (p67)
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 67 kD
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Fimbrin (p67), p67 extracted from contracted actin-myosin complex from starved cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: PA1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_1553688 
Additional Information: csA from strain AX2-214. Immunization used Alugel S. RRID:AB_1553688
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
210-183-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 210-183-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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