Clone ID / Name: 22/18
Anti - blastemal regeneration cell marker of the newt

Catalog Fields
Identity
Clone ID / Name: 22/18
Alternate Antibody Name: —
Antigen Name: blastemal regeneration cell marker of the newt
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: —
Entrez Gene ID: —
Antigen Sequence: —
Antigen Species: newt
Antigen Mol. Weight: —
Uniprot ID: —
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgM
Myeloma Strain: NS1/SP2
Antibody Registry / RRID: AB_531815
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: early to mid-bud stage blastemas
Immunogen Sequence: —
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: The immunization used 50-100 micrograms of protein for priming and for several boost for sacrificing. The origin of the antibody is notophthalmus viridescens. Works with Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates. RRID:AB_531815
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Axolotl, Newt
Predicted Reactivity: —
Depositor
Depositor Name: J.P. Brockes
Institution: Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch
Deposit Date: January 1, 1987
Depositor Notes: Western blot was unsuccessful. For immunohistology see papers of developing and regenerating limbs and normal embryos. The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
Recommended Applications
IHC
Each lab must optimize concentration per application and species. Full usage recommendations →
UniProt DataUniProtNo UniProt ID
▼No UniProt ID is available for this product.

