22/18

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$40.00
SKU: 22/18
View product citations for antibody 22/18 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: blastemal regeneration cell marker of the newt
Hybridoma Cells Available: Yes
Antigen Species: newt
Depositor: Brockes, J.P.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch
Positive Tested Species Reactivity: Axolotl, Newt
Depositors Notes: Western blot was unsuccessful. For immunohistology see papers of developing and regenerating limbs and normal embryos. The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: early to mid-bud stage blastemas
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1/SP2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531815 
Additional Information: The immunization used 50-100 micrograms of protein for priming and for several boost for sacrificing. The origin of the antibody is notophthalmus viridescens. Works with Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates. RRID:AB_531815
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22/18 was deposited to the DSHB by Brockes, J.P. (DSHB Hybridoma Product 22/18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration.
    Brockes JP
    Nature 308.5954 (1984 Mar 1-7): 67-9.

    Monoclonal antibodies to the cells of a regenerating limb.
    Brockes JP
    Journal of embryology and experimental morphology 89. (1985 Oct): 37-55.

    Glial growth factor and nerve-dependent proliferation in the regeneration blastema of Urodele amphibians.
    Kintner CR
    Cell 45.2 (1986 Apr 25): 301-6.

    A monoclonal antibody detects a difference in the cellular composition of developing and regenerating limbs of newts.
    Brockes JP
    Development (Cambridge, England) 99.4 (1987 Apr): 589-602.

    A monoclonal antibody stains myogenic cells in regenerating newt muscle.
    Carlson BM
    Development (Cambridge, England) 101.2 (1987 Oct): 267-77.

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