22B12B2

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$45.00 to $425.00
SKU: 22B12B2
View product citations for antibody 22B12B2 on CiteAb

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Available: 13

DSHB Data Sheet

Catalog Fields

Antigen: 30S ribosomal protein S3
Available to For-Profits: Yes
Antigen Species: E. coli K12 (strain PR-C10)
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Kahan, L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: E. coli
Depositors Institution: University of Wisconsin-Madison, Dept. of Biomolecular Chemistry
Antigen Molecular Weight: 25.9 kDa
Depositors Notes: The first publication is Journal of Protein Chemistry 9(2):159 (1990).
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: 30S subunit (S1-depleted)
Gene: rpsC
Alternate Antibody Name:
Alternate Gene Names: Y75_p3862
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: Yes
Uniprot ID: P0A7V3 
Epitope Location or Sequence: a.a. 1-21 of S3
Entrez Gene ID: 23846857 947814 
Immunogen Sequence:
Antibody Registry ID: AB_2618086 
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
Additional Information: Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation RRID:AB_2618086
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22B12B2 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 22B12B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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