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SKU: 22B12B2
View product citations for antibody 22B12B2 on CiteAb

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Available: 87

DSHB Data Sheet

Catalog Fields

Antigen: 30S ribosomal protein S3
Hybridoma Cells Available: Yes
Antigen Species: E. coli K12 (strain PR-C10)
Depositor: Kahan, L.
Isotype: MIgG1
Depositors Institution: University of Wisconsin-Madison
Host Species: mouse
Depositors Notes: The first publication is Journal of Protein Chemistry 9(2):159 (1990).
Positive Tested Species Reactivity: E. coli
Gene: rpsC
Antigen Molecular Weight: 25.9 kDa
Alternate Gene Names: Y75_p3862
Immunogen: 30S subunit (S1-depleted)
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: Yes
Uniprot ID: P0A7V3 
Epitope Location or Sequence: a.a. 1-21 of S3
Entrez Gene ID: 23846857 947814 
Additional Information: Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation RRID:AB_2618086
Antibody Registry ID: AB_2618086 
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22B12B2 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 22B12B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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