23-132-27

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$40.00
SKU: 23-132-27
View product citations for antibody 23-132-27 on CiteAb

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Available: 41

DSHB Data Sheet

Catalog Fields

Antigen: calmodulin
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium
Depositor: Gerisch, G.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 20 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: calA
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/01
Epitope Location or Sequence:
Uniprot ID: P02599 
Immunogen Sequence:
Entrez Gene ID: 8622104 
Additional Characterization:
Antibody Registry ID: AB_2069425 
Additional Information: Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases RRID:AB_2069425
Recommended Applications:  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23-132-27 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 23-132-27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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