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SKU: 232-238-8
View product citations for antibody 232-238-8 on CiteAb

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Available: 42

DSHB Data Sheet

Catalog Fields

Antigen: Cortexillin II
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Host Species: mouse
Gene: ctxB
Positive Tested Species Reactivity: Dictyostelium
Clonality: Monoclonal
Antigen Molecular Weight: 50 kDa
Additional Information: Actin-bundling protein. When linked to F-actin the actin filaments form preferentially anti-parallel bundles that associate into meshworks. Plays a major role in cytokinesis. Negatively regulates cortical localization of rapgap1 RRID:AB_10573804
Immunogen: N-terminally His-tagged cortexillin II
Myeloma Strain: PA1
Uniprot ID: Q550R2 
Entrez Gene ID: 8620736 
Antibody Registry ID: AB_10573804 
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
232-238-8 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 232-238-8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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