23A7 (anti NrCAM)

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SKU: 23A7 (anti NrCAM)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 23A7 (anti NrCAM)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528414 
Uniprot ID: P35331 
RRID:  
Entrez Gene ID: 396202 
Clonality: Monoclonal
Immunogen: Chicken spinal cord
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: NrCAM
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/20/2002
Antigen Molecular Weight: Apparent: 118 kDa
Depositor: Denburg, J.
Antigen Sequence:
Depositor Institution: University of Iowa, Department of Biological Sciences
Antigen Species: chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken
Additional Information: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Specifically involved in the development of optic fibres in the retina. RRID:AB_528414
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23A7 (anti NrCAM) was deposited to the DSHB by Denburg, J. (DSHB Hybridoma Product 23A7 (anti NrCAM))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

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