23A7 (anti NrCAM)

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SKU: 23A7 (anti NrCAM)
View product citations for antibody 23A7 (anti NrCAM) on CiteAb

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DSHB Data Sheet

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Antigen: NrCAM
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Denburg, J.
Isotype: MIgG1
Depositors Institution: Dept. of Biological Sciences, University of Iowa
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Chicken
Epitope Mapped: No
Antigen Molecular Weight: Apparent: 118 kDa
Additional Information: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Specifically involved in the development of optic fibres in the retina. RRID:AB_528414
Immunogen: Chicken spinal cord
Myeloma Strain: NS-1
Uniprot ID: P35331 
Entrez Gene ID: 396202 
Antibody Registry ID: AB_528414 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23A7 (anti NrCAM) was deposited to the DSHB by Denburg, J. (DSHB Hybridoma Product 23A7 (anti NrCAM))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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