23A7 (anti NrCAM)

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$40.00
SKU: 23A7 (anti NrCAM)
View product citations for antibody 23A7 (anti NrCAM) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NrCAM
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Denburg, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Dept. of Biological Sciences, University of Iowa
Positive Tested Species Reactivity: Chicken
Depositors Notes:
Antigen Molecular Weight: Apparent: 118 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Chicken spinal cord
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P35331 
Immunogen Sequence:
Entrez Gene ID: 396202 
Additional Characterization:  
Antibody Registry ID: AB_528414 
Additional Information: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Specifically involved in the development of optic fibres in the retina. RRID:AB_528414
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23A7 (anti NrCAM) was deposited to the DSHB by Denburg, J. (DSHB Hybridoma Product 23A7 (anti NrCAM))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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