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SKU: 23E9E7
View product citations for antibody 23E9E7 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: ribosome, 30S (E. coli)
Hybridoma Cells Available: Yes
Antigen Species: E. coli
Depositor: Kahan, L.
Isotype: MIgG1
Depositors Institution: University of Wisconsin-Madison, Dept. of Biomolecular Chemistry
Host Species: mouse
Gene: rpsC
Positive Tested Species Reactivity: E. coli
Clonality: Monoclonal
Immunogen: 30S ribosome
Epitope Mapped: No
Myeloma Strain: NS-1
Additional Information: RRID:AB_2618087
Uniprot ID: P0A7V3 
Entrez Gene ID: 23846857 947814 
Antibody Registry ID: AB_2618087 
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23E9E7 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 23E9E7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • All References
  • Initial Publication

    Epitope mapping of monoclonal antibodies to Escherichia coli ribosomal protein S3.
    Kahan L
    Journal of protein chemistry 9.2 (1990 Apr): 159-67.

    All References

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