241-438-1
Catalog Fields
Antigen: Cortexillin I
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG2b
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Host Species: mouse
Gene: ctxA
Positive Tested Species Reactivity: Dictyostelium
Clonality: Monoclonal
Antigen Molecular Weight: 50 kDa
Additional Information: RRID:AB_1553445
Immunogen: Cortexillin I
Myeloma Strain: PAIB3Ag8I
Uniprot ID: Q54HG2
Antibody Registry ID: AB_1553445
Recommended Applications: Function Blocking, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
241-438-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 241-438-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tail.
Gerisch G
Cell 86.4 (1996 Aug 23): 631-42.