Antigen: Aggregatibacter actinomycetemcomitans
Hybridoma Cells Available: No
Antigen Species: A. actinomycetemcomitans
Depositor: Gmuer, R.
Depositors Institution: University of Zuerich, Institute of Oral Biology
Host Species: mouse
Depositors Notes: Initial publication is only available in book form. Gmôr, R. (1995). Value of new serological probes for the study of putative periodontal pathogens. Carol Stream, IL. Quintessence Publishing Co, Inc. Works with A. actinomycetemcomitans bacteria (any serotype plus non-serotypable bacteria)
Positive Tested Species Reactivity: Aggregatibacter actinomycetemcomitans, gram-negative bacteria
Immunogen: Aggregatibacter actinomycetemcomitans , strains OMZ 535, OMZ 537, OMZ 540, OMZ 585
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag-14
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (email@example.com). RRID:AB_2618059
Antibody Registry ID: AB_2618059
Recommended Applications: Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
261AA2.2 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 261AA2.2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm.
Antimicrobial agents and chemotherapy 54.6 (2010 Jun): 2489-96.