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SKU: 26E4C7
View product citations for antibody 26E4C7 on CiteAb

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Available: 42

DSHB Data Sheet

Catalog Fields

Antigen: PTP-ER
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1
Depositors Institution: University of California-Berkeley
Host Species: mouse
Depositors Notes: Protein tyrosine phosphatase-ERK/Enhancer of Ras1
Positive Tested Species Reactivity: Drosophila
Gene: PTP-ER
Immunogen: ER2-5/PTP-ER (Protein Tyrosine Phosphatase Enhancer RasV12)
Clonality: Monoclonal
Myeloma Strain: P3XAg8.653
Epitope Mapped: No
Uniprot ID: Q9W2F3 
Additional Information: RRID:AB_579788
Entrez Gene ID: 37461 
Antibody Registry ID: AB_579788 
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
26E4C7 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 26E4C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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