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SKU: 28F12
View product citations for antibody 28F12 on CiteAb

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Available: 64

DSHB Data Sheet

Catalog Fields

Antigen: TSLP
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Farr, A.G .
Isotype: RIgG2a
Depositors Institution: University of Washington, Department of Biological Stucture
Host Species: rat
Depositors Notes: This antibody blocks TSLP activity in vitro and in vivo
Positive Tested Species Reactivity: Mouse
Gene: TSLP
Antigen Molecular Weight: ~15 kDa
Clonality: Monoclonal
Immunogen: TSLP
Additional Information: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. Can induce allergic inflammation by directly activating mast cells RRID:AB_1157894
Myeloma Strain: P3X63Ag8.653
Uniprot ID: Q9JIE6 
Entrez Gene ID: 53603 
Antibody Registry ID: AB_1157894 
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
28F12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 28F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    A mechanism for the initiation of allergen-induced T helper type 2 responses.
    Medzhitov R
    Nature immunology 9.3 (2008 Mar): 310-8.

    All References

    A mechanism for the initiation of allergen-induced T helper type 2 responses.
    Medzhitov R
    Nature immunology 9.3 (2008 Mar): 310-8.

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