(0)No Reviews yet
$45.00 to $425.00
SKU: 28F12
View product citations for antibody 28F12 on CiteAb

In Stock

Available: 46

DSHB Data Sheet

Catalog Fields

Antigen: TSLP
Available to For-Profits: Yes
Antigen Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG2a
Depositor: Farr, A.G .
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Mouse
Depositors Institution: University of Washington, Department of Biological Stucture
Antigen Molecular Weight: ~15 kDa
Depositors Notes: This antibody blocks TSLP activity in vitro and in vivo
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: TSLP
Gene: TSLP
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: No
Uniprot ID: Q9JIE6 
Epitope Location or Sequence:
Entrez Gene ID: 53603 
Immunogen Sequence:
Antibody Registry ID: AB_1157894 
Additional Characterization:  
Recommended Applications: Function Blocking
Additional Information: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. Can induce allergic inflammation by directly activating mast cells RRID:AB_1157894
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
28F12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 28F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • FB References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review