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$45.00 to $425.00
SKU: 28F12
View product citations for antibody 28F12 on CiteAb

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Available: 46

DSHB Data Sheet

Catalog Fields

Antigen: TSLP
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Farr, A.G .
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: University of Washington, Department of Biological Stucture
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody blocks TSLP activity in vitro and in vivo
Antigen Molecular Weight: ~15 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TSLP
Immunogen: TSLP
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q9JIE6 
Immunogen Sequence:
Entrez Gene ID: 53603 
Additional Characterization:  
Antibody Registry ID: AB_1157894 
Additional Information: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. Can induce allergic inflammation by directly activating mast cells RRID:AB_1157894
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
28F12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 28F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • FB References
  • All References
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