2A4

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SKU: 2A4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 2A4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: HK1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528280 
Uniprot ID: P05708 
RRID:  
Entrez Gene ID: 25058 
Clonality: Monoclonal
Immunogen: Type I isozyme of rat hexokinase
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: hexokinase (Type I isozyme)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/10/2002
Antigen Molecular Weight:
Depositor: Wilson, J.E.
Antigen Sequence:
Depositor Institution: Michigan State University
Antigen Species: rat
Depositor Notes: Monoclonal 2A4 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. The epitope recognized is a conformational epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178].
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528280
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A4 was deposited to the DSHB by Wilson, J.E. (DSHB Hybridoma Product 2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

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