2A4
Catalog Fields
Clone ID/Product Name: 2A4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: HK1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 25058
Clonality: Monoclonal
Immunogen: Type I isozyme of rat hexokinase
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: hexokinase (Type I isozyme)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/10/2002
Antigen Molecular Weight:
Depositor: Wilson, J.E.
Antigen Sequence:
Depositor Institution: Michigan State University
Antigen Species: rat
Depositor Notes: Monoclonal 2A4 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. The epitope recognized is a conformational epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178].
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528280
Recommended Applications: ELISA, Function Blocking