Antibody Name: 2A4
Antigen:hexokinase (Type I isozyme)
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: SP2/0-Ag14
Antibody Registry / RRID: AB_528280
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Type I isozyme of rat hexokinase
Immunogen Sequence: —
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528280
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Rat
Predicted Reactivity: —
Depositor
Depositor Name: J.E. Wilson
Institution: Michigan State University
Deposit Date: December 10, 2002
Depositor Notes: Monoclonal 2A4 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. The epitope recognized is a conformational epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178].
Recommended Applications
ELISA
Function Blocking
Each lab must optimize concentration per application and species. Full usage recommendations →
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