2C2
Catalog Fields
Antigen: collagen type IX
Hybridoma Cells Available:
Antigen Species: chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Dept. of Cell Biology, University of Alabama
Positive Tested Species Reactivity: Chicken
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Gene:
Immunogen: Type IX collagen from adult chicken sternum
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63A8.653
Epitope Location or Sequence: NC2 domain, C-terminus of HMW fragment
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528171
Additional Information: RRID:AB_528171
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 2C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
5 References
Initial Publication
Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
Mayne R
The Journal of cell biology 101.3 (1985 Sep): 814-23.