2C2

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$40.00
SKU: 2C2-s
View product citations for antibody 2C2 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: collagen type IX
Hybridoma Cells Available: No
Antigen Species: chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Dept. of Cell Biology, University of Alabama
Positive Tested Species Reactivity: Chicken, Quail
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Type IX collagen from adult chicken sternum
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63A8.653
Epitope Location or Sequence: NC2 domain, C-terminus of HMW fragment
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528171 
Additional Information: RRID:AB_528171
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 2C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Developmental evolution of the distal ankle in the dinosaur-bird transition.
    Vargas AO
    Journal of experimental zoology. Part B, Molecular and developmental evolution . (2020 Dec 31): .

    WB References
    IHC References
    FB References
    Epitope Map References
    All References

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Developmental evolution of the distal ankle in the dinosaur-bird transition.
    Vargas AO
    Journal of experimental zoology. Part B, Molecular and developmental evolution . (2020 Dec 31): .

    Structural and functional comparison of type IX collagen-proteoglycan from chicken cartilage and vitreous humor.
    Mayne R
    The Journal of biological chemistry 266.8 (1991 Mar 15): 4752-7.

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