2C2

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SKU: 2C2-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 2C2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528171 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Type IX collagen from adult chicken sternum
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63A8.653
Epitope Mapped: Yes
Antigen Name: collagen type IX
Epitope Location or Sequence: NC2 domain, C-terminus of HMW fragment
Alternate Antigen Name:
Deposit Date: 5/20/1993
Antigen Molecular Weight:
Depositor: Mayne, R.
Antigen Sequence:
Depositor Institution: University of Alabama at Birmingham
Antigen Species: chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chicken, Quail
Additional Information: RRID:AB_528171
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C2 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 2C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Developmental evolution of the distal ankle in the dinosaur-bird transition.
    Vargas AO
    Journal of experimental zoology. Part B, Molecular and developmental evolution 338.1-2 (2022 Jan): 119-128.

    WB References
    IHC References
    FB References
    Epitope Map References
    All References

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Developmental evolution of the distal ankle in the dinosaur-bird transition.
    Vargas AO
    Journal of experimental zoology. Part B, Molecular and developmental evolution 338.1-2 (2022 Jan): 119-128.

    Structural and functional comparison of type IX collagen-proteoglycan from chicken cartilage and vitreous humor.
    Mayne R
    The Journal of biological chemistry 266.8 (1991 Mar 15): 4752-7.

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