2C4-C2

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$45.00 to $425.00
SKU: 2C4-C2
View product citations for antibody 2C4-C2 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: Regenerating blastema; neurons, intestinal cells, anterior cells
Available to For-Profits: Yes
Antigen Species: Schmidtea mediterranea
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Zayas, R.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Schmidtea mediterranea
Depositors Institution: San Diego State University
Antigen Molecular Weight:
Depositors Notes: Strongly stains the regenerating blastema. Specific to ventral cells with the morphology of bipolar neurons found in the region anterior to the pharynx region to the posterior end of animal; also labels large peri-pharyngeal cells, and small unidentified cells in the head region and the regenerating blastema
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
Gene:
Alternate Antibody Name: 2C4
Alternate Gene Names:
Alternate Antigen Name:
Clonality:
Myeloma Strain: F0
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2619609 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C4-C2 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 2C4-C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    IF References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    All References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

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