2C6

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$40.00
SKU: 2C6
View product citations for antibody 2C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: alpha-2-macroglobulin receptor
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes: Cell membrane coated pit and receptosome glycoprotein
Antigen Molecular Weight: 180 - 190 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: NIH/3T3 mouse embryo fibroblast tissue culture cell membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3-NSI/1-Ag4-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528078 
Additional Information: Additional publication: August, J.T., Hughes, E.N., and Mengod, G. (1981). Murine cell surface glycoproteins: macrophage differentiation antigens identified and purified with monoclonal antibodies. In: Expression of Differentiated Functions in Cancer Cells (R.P. Revoltella, ed.), pp. 79-91, Raven Press, NY. RRID:AB_528078
Recommended Applications: Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C6 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product 2C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    IHC References
    IP References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Coprecipitation of heat shock proteins with a cell surface glycoprotein.
    August JT
    Proceedings of the National Academy of Sciences of the United States of America 79.7 (1982 Apr): 2305-9.

    All References

    Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.
    August JT
    The Journal of cell biology 97.2 (1983 Aug): 533-41.

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Coprecipitation of heat shock proteins with a cell surface glycoprotein.
    August JT
    Proceedings of the National Academy of Sciences of the United States of America 79.7 (1982 Apr): 2305-9.

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