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SKU: 2D5-s
View product citations for antibody 2D5 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: MHC class II
Depositor: Miller, M.M.
Antigen Species: chicken
Depositors Institution: Beckman Research Institute of the City of Hope
Isotype: MIgG3
Clonality: Monoclonal
Host Species: mouse
Additional Information: RRID:AB_528352
Positive Tested Species Reactivity: Chicken
Antigen Molecular Weight: 30 and 32 kDa
Immunogen: Ficoll gradient purified spleen cells from chicken
Myeloma Strain: SP2/0 Ag14
Antibody Registry ID: AB_528352 
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2D5 was deposited to the DSHB by Miller, M.M. (DSHB Hybridoma Product 2D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    All References

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