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SKU: 2D7F8
View product citations for antibody 2D7F8 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Protein tyrosine phosphatase ERK
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of California at Berkeley
Host Species: mouse
Gene: PTP-ER
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: Dmel_CG9856
Antigen Molecular Weight: 152.9 kDa
Clonality: Monoclonal
Myeloma Strain: P3Xag8.653
Additional Information: RRID:AB_579789. FBgn0016641.
Uniprot ID: Q9W2F3 
Entrez Gene ID: 37461 
Antibody Registry ID: AB_579789 
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2D7F8 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 2D7F8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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