2E7 PI-9

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SKU: 2E7 PI-9-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 2E7 PI-9
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: SERPINB9
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2936808 
Uniprot ID: P50453 
Entrez Gene ID: 5272 
Clonality: Monoclonal
Immunogen: Recombinant protein
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: SERPINB9 / PI-9
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/26/2023
Antigen Molecular Weight: 42.4 kDa
Depositor: Bird, P.I.
Antigen Sequence:
Depositor Institution: Monash University, Melbourne, AU
Antigen Species: Human
Depositor Notes: Serpinb9 is an intracellular inhibitor of the cytoxic protease granzyme B found in (but not limited to) CD8+ T cells, natural killer cells, mast cells and CD8+ dendritic cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: mAb 2E7 recognizes an unique epitope not present on related famil B / ovalbumin serpins.It does not recognise denatured protein.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E7 PI-9 was deposited to the DSHB by Bird, P.I. (DSHB Hybridoma Product 2E7 PI-9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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