2G9
Catalog Fields
Clone ID/Product Name: 2G9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Lsamp
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2138210
Uniprot ID: Q62813
Entrez Gene ID: 29561
Clonality: Monoclonal
Immunogen: Adult Rat hippocampal crude membrane preparation
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Lsamp (Lamp)
Epitope Location or Sequence: First Ig domain (aa 1-94 of mature protein)
Alternate Antigen Name:
Deposit Date: 1/23/2008
Antigen Molecular Weight: Predicted: 32 kDa (mature form); Apparent: 64-68 kDa
Depositor: Levitt, P.R.
Antigen Sequence:
Depositor Institution: Vanderbilt Kennedy Ctr. for Research on Human Development
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Cat, Fish, Human, Mouse, Primate, Rat
Additional Information: Lsamp is a GPI-anchored glycoprotein.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2G9 was deposited to the DSHB by Levitt, P.R. (DSHB Hybridoma Product 2G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
A monoclonal antibody to limbic system neurons.
Levitt P
Science (New York, N.Y.) 223.4633 (1984 Jan 20): 299-301.