2G9

(0) No Reviews yet
$40.00
SKU: 2G9
View product citations for antibody 2G9 on CiteAb

In Stock

Available: 33

DSHB Data Sheet

Catalog Fields

Antigen: Lsamp (Lamp)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Levitt, P.R.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt Kennedy Ctr. for Research on Human Development
Positive Tested Species Reactivity: Bovine, Cat, Fish, Human, Mouse, Primate, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 32 kDa (mature form); Apparent: 64-68 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lsamp
Immunogen: Adult Rat hippocampal crude membrane preparation
Alternate Gene Names: Lamp
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: First Ig domain (aa 1-94 of mature protein)
Uniprot ID: Q62813 
Immunogen Sequence: Full length protein
Entrez Gene ID: 29561 
Additional Characterization:
Antibody Registry ID: AB_2138210 
Additional Information: Lsamp is a GPI-anchored glycoprotein.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2G9 was deposited to the DSHB by Levitt, P.R. (DSHB Hybridoma Product 2G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody to limbic system neurons.
    Levitt P
    Science (New York, N.Y.) 223.4633 (1984 Jan 20): 299-301.

    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    All References

    A unique membrane protein is expressed on early developing limbic system axons and cortical targets.
    Levitt P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.12 (1988 Dec): 4653-61.

    Developmental and regeneration-associated regulation of the limbic system associated membrane protein in explant cultures of the rat brain.
    Levitt P
    Neuroscience 28.2 (1989): 455-74.

    Isolation, biochemical characterization and ultrastructural analysis of the limbic system-associated membrane protein (LAMP), a protein expressed by neurons comprising functional neural circuits.
    Levitt P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 10.1 (1990 Jan): 73-90.

    Heterogeneous distribution of the limbic system-associated membrane protein in the caudate nucleus and substantia nigra of the cat.
    Levitt P
    Neuroscience 40.3 (1991): 725-33.

    Limbic system-associated membrane protein (LAMP) in primate amygdala and hippocampus.
    Parent A
    Hippocampus 6.5 (1996): 483-94.

    A monoclonal antibody to limbic system neurons.
    Levitt P
    Science (New York, N.Y.) 223.4633 (1984 Jan 20): 299-301.

    The limbic system-associated membrane protein is an Ig superfamily member that mediates selective neuronal growth and axon targeting.
    Levitt P
    Neuron 15.2 (1995 Aug): 287-97.

    The limbic system-associated membrane protein (LAMP) selectively mediates interactions with specific central neuron populations.
    Levitt P
    Development (Cambridge, England) 121.4 (1995 Apr): 1161-72.

    Distribution of limbic system-associated membrane protein immunoreactivity in primate basal ganglia.
    Parent A
    Neuroscience 69.1 (1995 Nov): 71-81.

    Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP.
    Salzer JL
    Journal of neurobiology 51.3 (2002 Jun 5): 190-204.

    Distinct domains of the limbic system-associated membrane protein (LAMP) mediate discrete effects on neurite outgrowth.
    Levitt P
    Molecular and cellular neurosciences 24.3 (2003 Nov): 725-40.

    A membrane glycoprotein associated with the limbic system mediates the formation of the septo-hippocampal pathway in vitro.
    Levitt P
    Neuron 3.5 (1989 Nov): 551-61.

    Membrane-associated molecules guide limbic and nonlimbic thalamocortical projections.
    Bolz J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.22 (1998 Nov 15): 9409-19.

    Developmental studies with a specific cell surface marker for limbic system neurons.
    Levitt P
    Progress in clinical and biological research 217A. (1986): 111-3.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review