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SKU: 31-1C10-s
View product citations for antibody 31-1C10 on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: Muscarinic Acetylcholine Receptor M2 (cardiac)
Hybridoma Cells Available: No
Antigen Species: Porcine
Depositor: Nathanson, N.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: U of Wash School of Medicine - Seattle
Positive Tested Species Reactivity: Porcine, Rat
Depositors Notes:
Antigen Molecular Weight: 60 kDa-75 kDa (glycosylated
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CHRM2
Immunogen: Purified mAChR
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP/2
Epitope Location or Sequence:
Uniprot ID: P06199 
Immunogen Sequence: Full length protein
Entrez Gene ID: 397498 
Additional Characterization:  
Antibody Registry ID: AB_2753215 
Additional Information:
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
31-1C10 was deposited to the DSHB by Nathanson, N.M. (DSHB Hybridoma Product 31-1C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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