31-1C10

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$40.00
SKU: 31-1C10-s
View product citations for antibody 31-1C10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Muscarinic Acetylcholine Receptor M2 (cardiac)
Hybridoma Cells Available:
Antigen Species: Porcine
Depositor: Nathanson, N.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: U of Wash School of Medicine - Seattle
Positive Tested Species Reactivity: Porcine, Rat
Depositors Notes:
Antigen Molecular Weight: 60 kDa-75 kDa (glycosylated
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CHRM2
Immunogen: Purified mAChR
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP/2
Epitope Location or Sequence:
Uniprot ID: P06199 
Immunogen Sequence: Full length protein
Entrez Gene ID: 397498 
Additional Characterization:
Antibody Registry ID:
Additional Information:
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
31-1C10 was deposited to the DSHB by Nathanson, N.M. (DSHB Hybridoma Product 31-1C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
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