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SKU: 31-1C10-s
View product citations for antibody 31-1C10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Muscarinic Acetylcholine Receptor M2 (cardiac)
Available to For-Profits: Yes
Antigen Species: Porcine
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: Nathanson, N.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Porcine, Rat
Depositors Institution: University of Washington School of Medicine
Antigen Molecular Weight: 60 kDa-75 kDa (glycosylated
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified mAChR
Gene: CHRM2
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP/2
Epitope Mapped: No
Uniprot ID: P06199 
Epitope Location or Sequence:
Entrez Gene ID: 397498 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2753215 
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
31-1C10 was deposited to the DSHB by Nathanson, N.M. (DSHB Hybridoma Product 31-1C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
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