3B11 anti-grasshopper Fas I

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SKU: 3B11 anti-grasshopper Fas I

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 3B11 anti-grasshopper Fas I
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Fas1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_528314 
Uniprot ID: P10675 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Membrane preparations from connectives (axon bundles connecting the segmental ganglia) from 25 adult grasshoppers.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: fasciclin I (grasshopper)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Apparent: 70 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: grasshopper
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Grasshopper
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B11 anti-grasshopper Fas I was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 3B11 anti-grasshopper Fas I)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper.
    Goodman CS
    Cell 48.5 (1987 Mar 13): 745-55.

    Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.
    Goodman CS
    Proceedings of the National Academy of Sciences of the United States of America 85.14 (1988 Jul): 5291-5.

    Expression and structural studies of fasciclin I, an insect cell adhesion molecule.
    Bjorkman PJ
    The Journal of biological chemistry 268.2 (1993 Jan 15): 1448-55.

    Regeneration of olfactory afferent axons in the locust brain.
    Bicker G
    The Journal of comparative neurology 520.4 (2012 Mar 1): 679-93.

    Binding and internalization of Helicobacter pylori VacA via cellular lipid rafts in epithelial cells.
    Wang WC
    Biochemical and biophysical research communications 303.2 (2003 Apr 4): 640-4.

    A developmental study of enteric neuron migration in the grasshopper using immunological probes.
    Bicker G
    Developmental dynamics : an official publication of the American Association of Anatomists 238.11 (2009 Nov): 2837-49.

    Lazarillo expression reveals a subset of neurons contributing to the primary axon scaffold of the embryonic brain of the grasshopper Schistocerca gregaria.
    Boyan G
    The Journal of comparative neurology 419.3 (2000 Apr 10): 394-405.

    Developmental expression of cell recognition molecules in the mushroom body and antennal lobe of the locust Locusta migratoria.
    Bicker G
    The Journal of comparative neurology 520.9 (2012 Jun 15): 2021-40.

    Commissure formation in the embryonic insect brain.
    Hirth F
    Arthropod structure & development 32.1 (2003 Aug): 61-77.

    WB References

    Binding and internalization of Helicobacter pylori VacA via cellular lipid rafts in epithelial cells.
    Wang WC
    Biochemical and biophysical research communications 303.2 (2003 Apr 4): 640-4.

    IHC References

    Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper.
    Goodman CS
    Cell 48.5 (1987 Mar 13): 745-55.

    Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset of neurons in the grasshopper embryo.
    Bastiani MJ
    Development (Cambridge, England) 121.1 (1995 Jan): 123-34.

    Disruption of pioneer growth cone guidance in vivo by removal of glycosyl-phosphatidylinositol-anchored cell surface proteins.
    Bentley D
    Development (Cambridge, England) 114.2 (1992 Feb): 507-19.

    Requirement of RNA synthesis for pathfinding by growing axons.
    Bastiani MJ
    The Journal of comparative neurology 357.1 (1995 Jun 19): 52-64.

    Commissure formation in the embryonic insect brain.
    Hirth F
    Arthropod structure & development 32.1 (2003 Aug): 61-77.

    Embryonic development and evolutionary origin of the Orthopteran auditory organs.
    Reichert H
    Journal of neurobiology 21.4 (1990 Jun): 592-610.

    IP References

    Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper.
    Goodman CS
    Cell 48.5 (1987 Mar 13): 745-55.

    Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.
    Goodman CS
    Proceedings of the National Academy of Sciences of the United States of America 85.14 (1988 Jul): 5291-5.

    Lachesin: an immunoglobulin superfamily protein whose expression correlates with neurogenesis in grasshopper embryos.
    Bastiani MJ
    Development (Cambridge, England) 118.2 (1993 Jun): 509-22.

    The restricted spatial and temporal expression of a nervous-system-specific antigen involved in axon outgrowth during development of the grasshopper.
    Bastiani MJ
    Development (Cambridge, England) 111.4 (1991 Apr): 881-93.

    Neural-specific carbohydrate moiety shared by many surface glycoproteins in Drosophila and grasshopper embryos.
    Goodman CS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4137-44.

    FB References
    FACS References

    Expression and structural studies of fasciclin I, an insect cell adhesion molecule.
    Bjorkman PJ
    The Journal of biological chemistry 268.2 (1993 Jan 15): 1448-55.

    All References

    Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper.
    Goodman CS
    Cell 48.5 (1987 Mar 13): 745-55.

    Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset of neurons in the grasshopper embryo.
    Bastiani MJ
    Development (Cambridge, England) 121.1 (1995 Jan): 123-34.

    Disruption of pioneer growth cone guidance in vivo by removal of glycosyl-phosphatidylinositol-anchored cell surface proteins.
    Bentley D
    Development (Cambridge, England) 114.2 (1992 Feb): 507-19.

    Requirement of RNA synthesis for pathfinding by growing axons.
    Bastiani MJ
    The Journal of comparative neurology 357.1 (1995 Jun 19): 52-64.

    Commissure formation in the embryonic insect brain.
    Hirth F
    Arthropod structure & development 32.1 (2003 Aug): 61-77.

    Embryonic development and evolutionary origin of the Orthopteran auditory organs.
    Reichert H
    Journal of neurobiology 21.4 (1990 Jun): 592-610.

    Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.
    Goodman CS
    Proceedings of the National Academy of Sciences of the United States of America 85.14 (1988 Jul): 5291-5.

    Expression and structural studies of fasciclin I, an insect cell adhesion molecule.
    Bjorkman PJ
    The Journal of biological chemistry 268.2 (1993 Jan 15): 1448-55.

    Regeneration of olfactory afferent axons in the locust brain.
    Bicker G
    The Journal of comparative neurology 520.4 (2012 Mar 1): 679-93.

    Binding and internalization of Helicobacter pylori VacA via cellular lipid rafts in epithelial cells.
    Wang WC
    Biochemical and biophysical research communications 303.2 (2003 Apr 4): 640-4.

    A developmental study of enteric neuron migration in the grasshopper using immunological probes.
    Bicker G
    Developmental dynamics : an official publication of the American Association of Anatomists 238.11 (2009 Nov): 2837-49.

    Lazarillo expression reveals a subset of neurons contributing to the primary axon scaffold of the embryonic brain of the grasshopper Schistocerca gregaria.
    Boyan G
    The Journal of comparative neurology 419.3 (2000 Apr 10): 394-405.

    Developmental expression of cell recognition molecules in the mushroom body and antennal lobe of the locust Locusta migratoria.
    Bicker G
    The Journal of comparative neurology 520.9 (2012 Jun 15): 2021-40.

    Lachesin: an immunoglobulin superfamily protein whose expression correlates with neurogenesis in grasshopper embryos.
    Bastiani MJ
    Development (Cambridge, England) 118.2 (1993 Jun): 509-22.

    The restricted spatial and temporal expression of a nervous-system-specific antigen involved in axon outgrowth during development of the grasshopper.
    Bastiani MJ
    Development (Cambridge, England) 111.4 (1991 Apr): 881-93.

    Neural-specific carbohydrate moiety shared by many surface glycoproteins in Drosophila and grasshopper embryos.
    Goodman CS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4137-44.

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