Antigen: Windpipe protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Antibody's specificity characterized using Bio-Rad's Bio-Plex 200 system. It does not show binding activity against mCD8.
Predicted Species Reactivity:
Immunogen: wdp XC-mCD8?
Alternate Gene Names: Dmel_CG3413
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: Q9W266
Immunogen Sequence: dWindpipe extracellular domain (a.a. 10-465) hFC fusion; mCD8 alpha: 28-225
Entrez Gene ID: 37548
Antibody Registry ID: AB_2753229
Additional Information: The Protein G-purified antibody was used to stain Drosophila stage 16 embryos. Staining pattern matches with published wdp transcript expression patterns. Western blot application at ~0.1 ng/ul. FBgn0034718
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B7B5 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3B7B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.