Antigen: Windpipe protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Depositor: Zinn, K.
Host Species: mouse
Positive Tested Species Reactivity: Drosophila
Depositors Institution: California Institute of Technology
Antigen Molecular Weight: 73.1 kDa
Depositors Notes: Antibody's specificity characterized using Bio-Rad's Bio-Plex 200 system. It does not show binding activity against mCD8.
Immunogen: wdp XC-mCD8?
Alternate Antibody Name:
Alternate Gene Names: Dmel_CG3413
Alternate Antigen Name:
Myeloma Strain: HL-1
Epitope Mapped: No
Uniprot ID: Q9W266
Epitope Location or Sequence:
Entrez Gene ID: 37548
Immunogen Sequence: dWindpipe extracellular domain (a.a. 10-465) hFC fusion; mCD8 alpha: 28-225
Antibody Registry ID: AB_2753229
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: The Protein G-purified antibody was used to stain Drosophila stage 16 embryos. Staining pattern matches with published wdp transcript expression patterns. Western blot application at ~0.1 ng/ul. FBgn0034718
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3B7B5 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 3B7B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).