3H1
Catalog Fields
Antigen: keratan sulfate (brain)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.U. / Margolis, R.K.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution:
Positive Tested Species Reactivity:
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name: phosphacan-KS
Clonality:
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID:
Additional Information:
Recommended Applications:
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H1 was deposited to the DSHB by Margolis, R.U. / Margolis, R.K. (DSHB Hybridoma Product 3H1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
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