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SKU: 4-34.10
View product citations for antibody 4-34.10 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: cAMP-specific phosphodieserase PDE4A4
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Bolger, G.B.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Alabama at Birmingham
Positive Tested Species Reactivity: Human, Rat
Depositors Notes:
Antigen Molecular Weight: 97 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PDE4A
Immunogen: Peptide
Alternate Gene Names: DPDE2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P27815 
Immunogen Sequence: a.a. 617-635 GDERSRETPESDRAN
Entrez Gene ID: 5141 
Additional Characterization:  
Antibody Registry ID: AB_2617203 
Additional Information: RRID:AB_2617203
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4-34.10 was deposited to the DSHB by Bolger, G.B. (DSHB Hybridoma Product 4-34.10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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