40BI3.2.1
Catalog Fields
Clone ID/Product Name: 40BI3.2.1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2618070
Clonality: Monoclonal
Immunogen: Prevotella intermedia, strain OMZ 248
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Prevotella intermedia (cell surface antigen)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/1/2011
Antigen Molecular Weight:
Depositor: Gmuer, R.
Antigen Sequence:
Depositor Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Species: Prevotella intermedia
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Prevotella intermedia
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40BI3.2.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 40BI3.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies.
Guggenheim B
Infection and immunity 42.2 (1983 Nov): 459-70.