421AG1

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$40.00
SKU: 421AG1-s
View product citations for antibody 421AG1 on CiteAb

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Available: 8

DSHB Data Sheet

Catalog Fields

Antigen: Actinomyces naeslundii and oris
Hybridoma Cells Available: No
Antigen Species: A. gerencseriae, bacteria from caries
Depositor: Gmuer, R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: ZZMK, University of Zuerich
Positive Tested Species Reactivity: Actinomyces naeslundii, Actinomyces oris
Depositors Notes: Species Specificity note: Actinomyces naeslundii (all strains tested), and Actinomyces oris (most strains tested)
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Recommended Applications: ELISA, Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
421AG1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 421AG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Gingival crevice microbiota from Chinese patients with gingivitis or necrotizing ulcerative gingivitis.
    Guggenheim B
    European journal of oral sciences 112.1 (2004 Feb): 33-41.

    All References

    Gingival crevice microbiota from Chinese patients with gingivitis or necrotizing ulcerative gingivitis.
    Guggenheim B
    European journal of oral sciences 112.1 (2004 Feb): 33-41.

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