455-2A4

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$40.00
SKU: 455-2A4
View product citations for antibody 455-2A4 on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: Transcription factor elt-2
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: McGhee, James D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Calgary
Positive Tested Species Reactivity: C. elegans
Depositors Notes: The transcription factor ELT-2 binds DNA GATA domains and controls C. elegans intestinal development. See PMID 19111532.
Antigen Molecular Weight: 47.1 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: elt-2
Immunogen: Recombinant His-tagged ELT-2- fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/MIL6
Epitope Location or Sequence:
Uniprot ID: Q10655 
Immunogen Sequence: Full length protein
Entrez Gene ID: 181250 
Additional Characterization:
Antibody Registry ID: AB_2618114 
Additional Information: RRID:AB_2618114
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
455-2A4 was deposited to the DSHB by McGhee, James D. (DSHB Hybridoma Product 455-2A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    The function and regulation of the GATA factor ELT-2 in the C. elegans endoderm.
    McGhee JD
    Development (Cambridge, England) 143.3 (2016 Feb 1): 483-91.

    IF References

    The function and regulation of the GATA factor ELT-2 in the C. elegans endoderm.
    McGhee JD
    Development (Cambridge, England) 143.3 (2016 Feb 1): 483-91.

    WB References

    The function and regulation of the GATA factor ELT-2 in the C. elegans endoderm.
    McGhee JD
    Development (Cambridge, England) 143.3 (2016 Feb 1): 483-91.

    IP References

    The function and regulation of the GATA factor ELT-2 in the C. elegans endoderm.
    McGhee JD
    Development (Cambridge, England) 143.3 (2016 Feb 1): 483-91.

    All References

    The function and regulation of the GATA factor ELT-2 in the C. elegans endoderm.
    McGhee JD
    Development (Cambridge, England) 143.3 (2016 Feb 1): 483-91.

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