4B12

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$40.00
SKU: 4B12-s
View product citations for antibody 4B12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, type III repeat 10, function blocking
Hybridoma Cells Available: No
Antigen Species: Xenopus laevis
Depositor: DeSimone, D.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Xenopus laevis
Depositors Notes: This antibody blocks cell attachment to fibronectin.
Antigen Molecular Weight: Predicted (multiple isoforms):up to 270 kDa; Apparent: 220 kDa (multiple isoforms)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: fn1
Immunogen: Recombinant GST/FN (type III repeats 9 to 11) fusion protein
Alternate Gene Names: FN, cig, finc, lets, msf
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/O-Ag 14
Epitope Location or Sequence: Type III repeat 10
Uniprot ID: Q91740 
Immunogen Sequence: Partial protein
Entrez Gene ID: 397744 
Additional Characterization:  
Antibody Registry ID: AB_2721945 
Additional Information: RRID:AB_2721945
Recommended Applications: Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4B12 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product 4B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
    DeSimone DW
    Development (Cambridge, England) 122.9 (1996 Sep): 2873-83.

    IF References
    WB References
    FB References
    Epitope Map References

    Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
    DeSimone DW
    Development (Cambridge, England) 122.9 (1996 Sep): 2873-83.

    All References

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