Antibody Name: 4B12
Antigen:fibronectin, type III repeat 10, function blocking
Fluorescent microscope image of the animal cap from a stage 12 Xenopus laevis embryo labeled with DSHB antibody 4B12. PMID: 26605543, Fig. 4D.
Catalog Fields
Identity
Clone ID / Antibody Name: 4B12
Alternate Antibody Name: —
Antigen Name: fibronectin, type III repeat 10, function blocking
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: fn1
Entrez Gene ID: 397744
Antigen Sequence: —
Antigen Species: Xenopus laevis
Antigen Mol. Weight: Predicted (multiple isoforms):up to 270 kDa; Apparent: 220 kDa (multiple isoforms)
Uniprot ID: Q91740
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: Sp2/O-Ag 14
Antibody Registry / RRID: AB_2721945
Available to For-Profits: Yes
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Recombinant GST/FN (type III repeats 9 to 11) fusion protein
Immunogen Sequence: Partial protein
Epitope Mapped: Yes
Epitope Location / Seq: Type III repeat 10
Clone: —
Additional Info: RRID:AB_2721945
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Xenopus laevis
Predicted Reactivity: —
Depositor
Depositor Name: D.W. DeSimone
Institution: University of Virginia
Deposit Date: June 23, 2015
Depositor Notes: This antibody blocks cell attachment to fibronectin.
Recommended Applications
Function Blocking
IF
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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