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SKU: 4B8-H5-D6
View product citations for antibody 4B8-H5-D6 on CiteAb

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Available: 32

DSHB Data Sheet

Catalog Fields

Antigen: inturned protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Adler, P.N.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Mild fixation is required for good immunostaining of In in tissues
Antigen Molecular Weight: 98.1 kd
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: in
Immunogen: E. coli Recombinant protein fragment
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp 2/0 - Ag 14
Epitope Location or Sequence:
Uniprot ID: Q9VPH0 
Immunogen Sequence: a.a. 47-255
Entrez Gene ID: 40246 
Additional Characterization:  
Antibody Registry ID: AB_2721946 
Additional Information: RRID:AB_2721946
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4B8-H5-D6 was deposited to the DSHB by Adler, P.N. (DSHB Hybridoma Product 4B8-H5-D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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