4D6

(0) No Reviews yet
$40.00
SKU: 4D6-s
View product citations for antibody 4D6 on CiteAb

In Stock

Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Collagen, Type IX
Hybridoma Cells Available: No
Antigen Species: Chicken
Depositor: Mayne, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Alabama at Birmingham
Positive Tested Species Reactivity: Chick, Mouse
Depositors Notes: Hyaluronidase digestion (1 mg/ml at 37? in PBS pH 5.9 for 30 minutes) may be required for expose the 4D6 epitope.
Antigen Molecular Weight: 63.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: COL9A3
Immunogen: Purified pepsin-digested sterna
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63A8.653
Epitope Location or Sequence: Junction of NC4 and N-terminus of COL3 domains
Uniprot ID: P32017 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396242 
Additional Characterization:  
Antibody Registry ID: AB_528172 
Additional Information: RRID:AB_2267538
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D6 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 4D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Embryonic chicken cornea and cartilage synthesize type IX collagen molecules with different amino-terminal domains.
    Olsen BR
    Proceedings of the National Academy of Sciences of the United States of America 85.20 (1988 Oct): 7496-7500.

    Collagen type IX and developmentally regulated swelling of the avian primary corneal stroma.
    Linsenmayer TF
    Developmental dynamics : an official publication of the American Association of Anatomists 212.1 (1998 May): 27-37.

    Differential localization of collagen type IX isoform messenger RNAs during early ocular development.
    Beebe DC
    Investigative ophthalmology & visual science 35.2 (1994 Feb): 470-8.

    Effects of the mesonephros and insulin-like growth factor I on chondrogenesis of limb explants.
    Solursh M
    Developmental biology 156.2 (1993 Apr): 500-8.

    Cellular invasion and collagen type IX in the primary corneal stroma in vitro.
    Linsenmayer TF
    Developmental dynamics : an official publication of the American Association of Anatomists 201.3 (1994 Nov): 206-15.

    Age-dependent changes in the expression of matrix components in the mouse eye.
    Vuorio E
    Experimental eye research 72.4 (2001 Apr): 423-31.

    WB References
    IHC References

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Notochord of chick embryos secretes short-form type IX collagen prior to the onset of vertebral chondrogenesis.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 194.3 (1992 Jul): 169-76.

    Collagen types IX and X in the developing chick tibiotarsus: analyses of mRNAs and proteins.
    Schmid TM
    Development (Cambridge, England) 111.1 (1991 Jan): 191-6.

    Widespread distribution of type II collagen during embryonic chick development.
    Solursh M
    Developmental biology 131.2 (1989 Feb): 558-66.

    Independent deposition of collagen types II and IX at epithelial-mesenchymal interfaces.
    Linsenmayer TF
    Development (Cambridge, England) 105.1 (1989 Jan): 85-95.

    Vitreous humor of chicken contains two fibrillar systems: an analysis of their structure.
    Mayne R
    Journal of ultrastructure and molecular structure research 100.3 (1988 Sep): 224-34.

    ELISA References
    Epitope Map References
    All References

    Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
    Linsenmayer TF
    Developmental biology 128.2 (1988 Aug): 396-405.

    Differential co-expression of long and short form type IX collagen transcripts during avian limb chondrogenesis in ovo.
    Solursh M
    Development (Cambridge, England) 115.1 (1992 May): 169-79.

    Notochord of chick embryos secretes short-form type IX collagen prior to the onset of vertebral chondrogenesis.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 194.3 (1992 Jul): 169-76.

    Collagen types IX and X in the developing chick tibiotarsus: analyses of mRNAs and proteins.
    Schmid TM
    Development (Cambridge, England) 111.1 (1991 Jan): 191-6.

    Widespread distribution of type II collagen during embryonic chick development.
    Solursh M
    Developmental biology 131.2 (1989 Feb): 558-66.

    Independent deposition of collagen types II and IX at epithelial-mesenchymal interfaces.
    Linsenmayer TF
    Development (Cambridge, England) 105.1 (1989 Jan): 85-95.

    Vitreous humor of chicken contains two fibrillar systems: an analysis of their structure.
    Mayne R
    Journal of ultrastructure and molecular structure research 100.3 (1988 Sep): 224-34.

    Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
    Mayne R
    The Journal of cell biology 101.3 (1985 Sep): 814-23.

    Cell surface receptors transmit sufficient force to bend collagen fibrils.
    Loeser RF
    Experimental cell research 248.1 (1999 Apr 10): 294-305.

    Embryonic chicken cornea and cartilage synthesize type IX collagen molecules with different amino-terminal domains.
    Olsen BR
    Proceedings of the National Academy of Sciences of the United States of America 85.20 (1988 Oct): 7496-7500.

    Collagen type IX and developmentally regulated swelling of the avian primary corneal stroma.
    Linsenmayer TF
    Developmental dynamics : an official publication of the American Association of Anatomists 212.1 (1998 May): 27-37.

    Differential localization of collagen type IX isoform messenger RNAs during early ocular development.
    Beebe DC
    Investigative ophthalmology & visual science 35.2 (1994 Feb): 470-8.

    Effects of the mesonephros and insulin-like growth factor I on chondrogenesis of limb explants.
    Solursh M
    Developmental biology 156.2 (1993 Apr): 500-8.

    Cellular invasion and collagen type IX in the primary corneal stroma in vitro.
    Linsenmayer TF
    Developmental dynamics : an official publication of the American Association of Anatomists 201.3 (1994 Nov): 206-15.

    Age-dependent changes in the expression of matrix components in the mouse eye.
    Vuorio E
    Experimental eye research 72.4 (2001 Apr): 423-31.

    D-periodic distribution of collagen type IX along cartilage fibrils.
    Mayne R
    The Journal of cell biology 106.3 (1988 Mar): 991-7.

    Structural and functional comparison of type IX collagen-proteoglycan from chicken cartilage and vitreous humor.
    Mayne R
    The Journal of biological chemistry 266.8 (1991 Mar 15): 4752-7.

    The structure and macromolecular organization of type IX collagen in cartilage.
    Mayne R
    Annals of the New York Academy of Sciences 580. (1990): 1-7.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review