4D6
Catalog Fields
Clone ID/Product Name: 4D6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: COL9A3
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 396242
Clonality: Monoclonal
Immunogen: Purified pepsin-digested sterna
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63A8.653
Epitope Mapped: Yes
Antigen Name: Collagen, Type IX
Epitope Location or Sequence: Junction of NC4 and N-terminus of COL3 domains
Alternate Antigen Name:
Deposit Date: 5/20/1993
Antigen Molecular Weight: 63.0 kDa
Depositor: Mayne, R.
Antigen Sequence:
Depositor Institution: University of Alabama at Birmingham
Antigen Species: Chicken
Depositor Notes: Hyaluronidase digestion (1 mg/ml at 37? in PBS pH 5.9 for 30 minutes) may be required for expose the 4D6 epitope.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick, Mouse
Additional Information: RRID:AB_2267538
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D6 was deposited to the DSHB by Mayne, R. (DSHB Hybridoma Product 4D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
Monoclonal antibody against chicken type IX collagen: preparation, characterization, and recognition of the intact form of type IX collagen secreted by chondrocytes.
Mayne R
The Journal of cell biology 101.3 (1985 Sep): 814-23.