4F5

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$50.00
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SKU: 4F5-s

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Available: 12

DSHB Data Sheet

Catalog Fields

Product Name/ID: 4F5
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: FCGR2B
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_3065009 
Uniprot ID: P31994 
RRID:  
Entrez Gene ID: 2213 
Clonality: Monoclonal
Immunogen: E.coli recombinant domain
Clone:
Immunogen Sequence: Extracellular domain
Myeloma Strain: P3 X 63A8.653
Epitope Mapped: No
Antigen Name: Fc gamma receptor Iib
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 34.0 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Human
Depositor Notes: mAb 4F5 does not cross-react with Fc?RIIa. Hybridoma developed by R.P. Kimberly.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: Fc?RIIb is expressed on monocytes, neutrophils, dendritic cells and B cells.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4F5 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product 4F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    IF References

    Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses.
    Edberg JC
    Science translational medicine 5.216 (2013 Dec 18): 216ra175.

    WB References

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses.
    Edberg JC
    Science translational medicine 5.216 (2013 Dec 18): 216ra175.

    Immune opsonins modulate BLyS/BAFF release in a receptor-specific fashion.
    Edberg JC
    Journal of immunology (Baltimore, Md. : 1950) 181.2 (2008 Jul 15): 1012-8.

    IP References

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    ELISA References

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    FB References

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    FACS References

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses.
    Edberg JC
    Science translational medicine 5.216 (2013 Dec 18): 216ra175.

    All References

    Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses.
    Edberg JC
    Science translational medicine 5.216 (2013 Dec 18): 216ra175.

    Expression profile of FcgammaRIIb on leukocytes and its dysregulation in systemic lupus erythematosus.
    Kimberly RP
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 3272-80.

    Immune opsonins modulate BLyS/BAFF release in a receptor-specific fashion.
    Edberg JC
    Journal of immunology (Baltimore, Md. : 1950) 181.2 (2008 Jul 15): 1012-8.

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