4H2

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$40.00
SKU: 4H2-s
View product citations for antibody 4H2 on CiteAb

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Available: 17

DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, type III repeat 9
Hybridoma Cells Available:
Antigen Species: Xenopus laevis
Depositor: DeSimone, D.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Xenopus laevis
Depositors Notes: 4H2 has no known effect on function.
Antigen Molecular Weight: Predicted (multiple isoforms):up to 270 kDa; Apparent (reducing conditions): 220 kDa (multiple isoforms)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: fn1
Immunogen: Xenous plasma fibronectin
Alternate Gene Names: FN, cig, finc, lets, msf
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/O-Ag 14
Epitope Location or Sequence: Type III repeat 9
Uniprot ID: Q91740 
Immunogen Sequence: Full length protein
Entrez Gene ID: 397744 
Additional Characterization:
Antibody Registry ID: AB_2721949 
Additional Information: RRID:AB_2721949
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4H2 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product 4H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Cadherin adhesion, tissue tension, and noncanonical Wnt signaling regulate fibronectin matrix organization.
    DeSimone DW
    Developmental cell 16.3 (2009 Mar): 421-32.

    Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo.
    DeSimone DW
    Journal of cell science 116.Pt 11 (2003 Jun 1): 2333-43.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    A ligand-independent integrin β1 mechanosensory complex guides spindle orientation.
    Skourides PA
    Nature communications 7. (2016 Mar 8): 10899.

    An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis.
    Bello V
    Development (Cambridge, England) 141.23 (2014 Dec): 4569-79.

    FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm.
    DeSimone DW
    Developmental biology 394.2 (2014 Oct 15): 340-56.

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular and developmental biology. Animal 49.3 (2013 Mar): 220-9.

    Hedgehog activity controls opening of the primary mouth.
    Liu KJ
    Developmental biology 396.1 (2014 Dec 1): 1-7.

    Directional migration of leading-edge mesoderm generates physical forces: Implication in Xenopus notochord formation during gastrulation.
    Ueno N
    Developmental biology 382.2 (2013 Oct 15): 482-95.

    A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis.
    Skourides PA
    Development (Cambridge, England) 140.20 (2013 Oct): 4266-76.

    WB References
    Epitope Map References

    Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
    DeSimone DW
    Development (Cambridge, England) 122.9 (1996 Sep): 2873-83.

    All References

    Cadherin adhesion, tissue tension, and noncanonical Wnt signaling regulate fibronectin matrix organization.
    DeSimone DW
    Developmental cell 16.3 (2009 Mar): 421-32.

    Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo.
    DeSimone DW
    Journal of cell science 116.Pt 11 (2003 Jun 1): 2333-43.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    A ligand-independent integrin β1 mechanosensory complex guides spindle orientation.
    Skourides PA
    Nature communications 7. (2016 Mar 8): 10899.

    An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis.
    Bello V
    Development (Cambridge, England) 141.23 (2014 Dec): 4569-79.

    FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm.
    DeSimone DW
    Developmental biology 394.2 (2014 Oct 15): 340-56.

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular and developmental biology. Animal 49.3 (2013 Mar): 220-9.

    Hedgehog activity controls opening of the primary mouth.
    Liu KJ
    Developmental biology 396.1 (2014 Dec 1): 1-7.

    Directional migration of leading-edge mesoderm generates physical forces: Implication in Xenopus notochord formation during gastrulation.
    Ueno N
    Developmental biology 382.2 (2013 Oct 15): 482-95.

    A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis.
    Skourides PA
    Development (Cambridge, England) 140.20 (2013 Oct): 4266-76.

    Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
    DeSimone DW
    Development (Cambridge, England) 122.9 (1996 Sep): 2873-83.

    V(+)-fibronectin expression and localization prior to gastrulation in Xenopus laevis embryos.
    Wedlich D
    Mechanisms of development 44.2-3 (1993 Dec): 155-65.

    Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation.
    DeSimone DW
    The Journal of cell biology 134.1 (1996 Jul): 227-40.

    Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin.
    DeSimone DW
    Development (Cambridge, England) 128.18 (2001 Sep): 3635-47.

    Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
    DeSimone DW
    Current biology : CB 13.14 (2003 Jul 15): 1182-91.

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