4H2
Catalog Fields
Clone ID/Product Name: 4H2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: fn1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2721949
Uniprot ID: Q91740
Entrez Gene ID: 397744
Clonality: Monoclonal
Immunogen: Xenous plasma fibronectin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/O-Ag 14
Epitope Mapped: Yes
Antigen Name: fibronectin, type III repeat 9
Epitope Location or Sequence: Type III repeat 9
Alternate Antigen Name:
Deposit Date: 6/23/2015
Antigen Molecular Weight: Predicted (multiple isoforms):up to 270 kDa; Apparent (reducing conditions): 220 kDa (multiple isoforms)
Depositor: DeSimone, D.W.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Xenopus laevis
Depositor Notes: 4H2 has no known effect on function.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Xenopus laevis
Additional Information: RRID:AB_2721949
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4H2 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product 4H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation.
DeSimone DW
The Journal of cell biology 134.1 (1996 Jul): 227-40.
Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
DeSimone DW
Development (Cambridge, England) 122.9 (1996 Sep): 2873-83.