5-23
Catalog Fields
Antigen: paramyosin
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Epstein, H.F.
Isotype: MIgG1
Depositors Institution: University of Texas Medical Branch
Host Species: mouse
Depositors Notes: stains all muscle cells; A-bands of body wall muscles; C. elegans, paramyosins of other species; paramyosin encoded by the unc-15 gene; structural protein in muscle, thick filament component
Positive Tested Species Reactivity: C. elegans
Epitope Mapped: No
Antigen Molecular Weight: ~108 kDa
Myeloma Strain: S194/5.XX0.Bu.1
Antibody Registry ID: AB_528425
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5-23 was deposited to the DSHB by Epstein, H.F. (DSHB Hybridoma Product 5-23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Immunochemical localization of myosin heavy chain isoforms and paramyosin in developmentally and structurally diverse muscle cell types of the nematode Caenorhabditis elegans.
Epstein HF
The Journal of cell biology 105.6 Pt 1 (1987 Dec): 2763-70.