5-23

(0) No Reviews yet
$40.00
SKU: 5-23
View product citations for antibody 5-23 on CiteAb

In Stock

Available: 17

DSHB Data Sheet

Catalog Fields

Antigen: paramyosin
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Epstein, H.F.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Texas Medical Branch
Positive Tested Species Reactivity: C. elegans
Depositors Notes: stains all muscle cells; A-bands of body wall muscles; C. elegans, paramyosins of other species; paramyosin encoded by the unc-15 gene; structural protein in muscle, thick filament component
Antigen Molecular Weight: ~108 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: S194/5.XX0.Bu.1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528425 
Additional Information:
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5-23 was deposited to the DSHB by Epstein, H.F. (DSHB Hybridoma Product 5-23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review