5-23
Catalog Fields
Antigen: Paramyosin
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Epstein, H.F.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Texas Medical Branch
Positive Tested Species Reactivity: C. elegans
Depositors Notes: stains all muscle cells; A-bands of body wall muscles; C. elegans, paramyosins of other species; paramyosin encoded by the unc-15 gene; structural protein in muscle, thick filament component
Predicted Species Reactivity:
Gene: UNC-15
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: S194/5.XX0.Bu.1
Epitope Location or Sequence:
Uniprot ID: P10567
Immunogen Sequence:
Entrez Gene ID: 172491
Antibody Registry ID: AB_528425
Additional Information:
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5-23 was deposited to the DSHB by Epstein, H.F. (DSHB Hybridoma Product 5-23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Immunochemical localization of myosin heavy chain isoforms and paramyosin in developmentally and structurally diverse muscle cell types of the nematode Caenorhabditis elegans.
Epstein HF
The Journal of cell biology 105.6 Pt 1 (1987 Dec): 2763-70.