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SKU: 5-6

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 5-6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYO3
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2147425 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: myosin
Immunogen Sequence: encoded by the myo-3 gene, 1969 amino acids
Myeloma Strain: S194/5.XX0.Bu.1
Epitope Mapped: No
Antigen Name: myosin heavy chain A
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/22/2003
Antigen Molecular Weight: about 210 kDa
Depositor: Epstein, H.F.
Antigen Sequence:
Depositor Institution: University of Texas Medical Branch
Antigen Species: C. elegans
Depositor Notes: Specificity note: C. elegans, others at higher concentration of antibody. Cell binding note: central zone of A-bands of muscle cells in body wall and elsewhere; not in pharynx. Antigen note: myosin heavy chain near the head/rod junction (S2). Functional Effects: structural and motor protein in muscle
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5-6 was deposited to the DSHB by Epstein, H.F. (DSHB Hybridoma Product 5-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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