56-396-5

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$40.00
SKU: 56-396-5
View product citations for antibody 56-396-5 on CiteAb

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Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Myosin II
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium
Depositor: Gerisch, G.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max-Plank-Institut fuer Biochemie, Germany
Positive Tested Species Reactivity: Dictyostelium, Tardigrades
Depositors Notes: Antibody 56-396-5 binds the myosin tail near the head region and demonstrates strong inhibition of the actin-activated ATPase activity. This antibody does not inhibit myosin polymerization.
Antigen Molecular Weight: 243 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: mhcA
Immunogen: Plasma membrane fraction
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2-01
Epitope Location or Sequence: Myosin tail
Uniprot ID: P08799 
Immunogen Sequence: Full length protein
Entrez Gene ID: 8625606 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
56-396-5 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 56-396-5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.
    Noegel AA
    Scientific reports 5. (2015 Sep 28): 14437.

    The tail domain of myosin M catalyses nucleotide exchange on Rac1 GTPases and can induce actin-driven surface protrusions.
    Soldati T
    Traffic (Copenhagen, Denmark) 1.5 (2000 May): 399-410.

    Actin-Interacting Protein 1 Contributes to Intranuclear Rod Assembly in Dictyostelium discoideum.
    Müller-Taubenberger A
    Scientific reports 7. (2017 Jan 11): 40310.

    A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin.
    Noegel AA
    Proceedings of the National Academy of Sciences of the United States of America 111.1 (2014 Jan 7): E25-33.

    Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles.
    Manstein DJ
    Cellular and molecular life sciences : CMLS 68.16 (2011 Aug): 2751-67.

    Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow.
    Gerisch G
    Journal of cell science 110 ( Pt 2). (1997 Jan): 123-37.

    Three-dimensional patterns and redistribution of myosin II and actin in mitotic Dictyostelium cells.
    Gerisch G
    The Journal of cell biology 139.7 (1997 Dec 29): 1793-804.

    WB References
    IHC References
    IP References

    Rearrangement of cortex proteins constitutes an osmoprotective mechanism in Dictyostelium.
    Schuster SC
    The EMBO journal 18.15 (1999 Aug 2): 4241-9.

    The Ste20-like kinase SvkA of Dictyostelium discoideum is essential for late stages of cytokinesis.
    Schleicher M
    Journal of cell science 120.Pt 24 (2007 Dec 15): 4345-54.

    FB References
    Epitope Map References
    All References

    Cellular morphology of leg musculature in the water bear <i>Hypsibius exemplaris</i> (Tardigrada) unravels serial homologies.
    Mayer G
    Royal Society open science 6.10 (2019 Oct): 191159.

    Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.
    Noegel AA
    Scientific reports 5. (2015 Sep 28): 14437.

    The tail domain of myosin M catalyses nucleotide exchange on Rac1 GTPases and can induce actin-driven surface protrusions.
    Soldati T
    Traffic (Copenhagen, Denmark) 1.5 (2000 May): 399-410.

    Actin-Interacting Protein 1 Contributes to Intranuclear Rod Assembly in Dictyostelium discoideum.
    Müller-Taubenberger A
    Scientific reports 7. (2017 Jan 11): 40310.

    A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin.
    Noegel AA
    Proceedings of the National Academy of Sciences of the United States of America 111.1 (2014 Jan 7): E25-33.

    Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles.
    Manstein DJ
    Cellular and molecular life sciences : CMLS 68.16 (2011 Aug): 2751-67.

    Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow.
    Gerisch G
    Journal of cell science 110 ( Pt 2). (1997 Jan): 123-37.

    Three-dimensional patterns and redistribution of myosin II and actin in mitotic Dictyostelium cells.
    Gerisch G
    The Journal of cell biology 139.7 (1997 Dec 29): 1793-804.

    Rearrangement of cortex proteins constitutes an osmoprotective mechanism in Dictyostelium.
    Schuster SC
    The EMBO journal 18.15 (1999 Aug 2): 4241-9.

    A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement.
    Faix J
    Nature communications 6. (2015 Sep 29): 8496.

    EGF-like peptide-enhanced cell movement in Dictyostelium is mediated by protein kinases and the activity of several cytoskeletal proteins.
    O'Day DH
    Cellular signalling 24.9 (2012 Sep): 1770-80.

    Monoclonal antibodies binding to the tail of Dictyostelium discoideum myosin: their effects on antiparallel and parallel assembly and actin-activated ATPase activity.
    Gerisch G
    The Journal of cell biology 103.4 (1986 Oct): 1527-38.

    The Ste20-like kinase SvkA of Dictyostelium discoideum is essential for late stages of cytokinesis.
    Schleicher M
    Journal of cell science 120.Pt 24 (2007 Dec 15): 4345-54.

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