56-396-5
Catalog Fields
Antigen: Myosin heavy chain, Type II
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium
Depositor: Gerisch, G.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max-Planck-Institut fuer Biochemie, Cell Dynamics
Positive Tested Species Reactivity: Dictyostelium, Tardigrades
Depositors Notes: Antibody 56-396-5 binds the myosin tail near the head region and demonstrates strong inhibition of the actin-activated ATPase activity. This antibody does not inhibit myosin polymerization.
Predicted Species Reactivity:
Gene: mhcA
Immunogen: Plasma membrane fraction
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2-01
Epitope Location or Sequence: Myosin tail
Uniprot ID: P08799
Immunogen Sequence: Full length protein
Entrez Gene ID: 8625606
Antibody Registry ID: AB_10586638
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
56-396-5 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 56-396-5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
14 References
Initial Publication
Monoclonal antibodies binding to the tail of Dictyostelium discoideum myosin: their effects on antiparallel and parallel assembly and actin-activated ATPase activity.
Gerisch G
The Journal of cell biology 103.4 (1986 Oct): 1527-38.