5B1-E6

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$40.00
SKU: 5B1-E6
View product citations for antibody 5B1-E6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Protonephria, ciliated structures
Hybridoma Cells Available: Yes
Antigen Species: Schmidtea mediterranea
Depositor: Zayas, R.M.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Biology, San Diego State University
Positive Tested Species Reactivity: Schmidtea mediterranea
Depositors Notes: Specific to ciliated structures; prominently labels the ciliated portion of protonephridia, dorsal stripe of ciliated epithelial cells, and ciliated ventral epithelial cells
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
Alternate Gene Names:
Alternate Antibody Name: 5B1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8U.1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2619610 
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5B1-E6 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 5B1-E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    IF References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    All References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

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