5B7-3D9

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$40.00
SKU: 5B7-3D9
View product citations for antibody 5B7-3D9 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: Flightin (muscle protein 27)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't recognize all Flightin isoforms
Antigen Molecular Weight: 20.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: fln
Immunogen: Purified Z-discs
Alternate Gene Names: FTN, mp27, Dmel_CG7445
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P35554 
Immunogen Sequence:
Entrez Gene ID: 40185 
Additional Characterization:  
Antibody Registry ID: AB_2721950 
Additional Information: FBgn0005633 RRID:AB_2721950
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5B7-3D9 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 5B7-3D9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • All References
  • Initial Publication

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

    WB References

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

    All References

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

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