5B7-3D9

(0) No Reviews yet
$40.00
SKU: 5B7-3D9
View product citations for antibody 5B7-3D9 on CiteAb

In Stock

Available: 31

DSHB Data Sheet

Catalog Fields

Antigen: Flightin (muscle protein 27)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't recognize all Flightin isoforms
Antigen Molecular Weight: 20.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: fln
Immunogen: Purified Z-discs
Alternate Gene Names: FTN, mp27, Dmel_CG7445
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P35554 
Immunogen Sequence:
Entrez Gene ID: 40185 
Additional Characterization:
Antibody Registry ID: AB_2721950 
Additional Information: FBgn0005633 RRID:AB_2721950
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5B7-3D9 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 5B7-3D9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • All References
  • Initial Publication

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

    WB References

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

    All References

    Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.
    Pardue ML
    The Journal of cell biology 121.3 (1993 May): 587-98.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review