5C2
Catalog Fields
Clone ID/Product Name: 5C2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528284
Clonality: Monoclonal
Immunogen: Type I isozyme of rat hexokinase
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/O-Ag14
Epitope Mapped: No
Antigen Name: hexokinase (Type I isozyme)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/10/2002
Antigen Molecular Weight:
Depositor: Wilson, J.E.
Antigen Sequence:
Depositor Institution: Michigan State University
Antigen Species: rat
Depositor Notes: Antigen note: N-terminal half of the Type I isozyme of hexokinase from rat. The epitope recognized is a “conformational” (or “discontinuous”) epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 65-178]. Immunoblotting note: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. Specificity note: Monoclonal 5C2 recognizes an epitope in the N-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. Crossreactivity with Type I isozyme from other mammalian species has not been investigated.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information:
Recommended Applications: ELISA