5C3 (reovirus)
Catalog Fields
Antigen: reovirus sigma 3
Hybridoma Cells Available: Yes
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Depositor: Dermody, T.S.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh School of Medicine
Positive Tested Species Reactivity: Type 1 Lang (T1L), Type 3 Dearing (T3D)
Depositors Notes: Inhibits sigma1 binding to sialoglycophorin on red blood cells.
Predicted Species Reactivity:
Gene: S4
Immunogen: T3D intact virus
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3 Ag8.6.5.3
Epitope Location or Sequence: Epitope resides within sigma 3
Uniprot ID: P07939
Immunogen Sequence: Full length sequence
Entrez Gene ID: 8437031
Antibody Registry ID: AB_2617409
Additional Information: RRID:AB_2617409
Recommended Applications: ELISA, Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5C3 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 5C3 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Monoclonal antibodies to reovirus reveal structure/function relationships between capsid proteins and genetics of susceptibility to antibody action.
Tyler KL
Journal of virology 65.12 (1991 Dec): 6772-81.