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$45.00 to $425.00
SKU: 5G10
View product citations for antibody 5G10 on CiteAb

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Available: 13

DSHB Data Sheet

Catalog Fields

Antigen: CD107/ LEP100/LAMP-1
Available to For-Profits: No
Antigen Species: Chick
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Reichardt, L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chick, Rat
Depositors Institution: University of California, San Francisco
Antigen Molecular Weight: 95-105 kDa 48 kDa core protein
Depositors Notes: The antigen is also known as lysosome-associated membrane glycoprotein 1 (LAMP-1) or lysosome membrane glycoprotein LEP100. This antibody clone is also known in the literature as ChCD107.
Predicted Species Reactivity:  
Human Protein Atlas:  
Gene: LAMP1
Alternate Antibody Name: CD107
Alternate Gene Names: LEP100
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: P05300 
Epitope Location or Sequence:
Entrez Gene ID: 396220 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_531769 
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5G10 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product 5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • FACS References
  • All References
  • Initial Publication

    Identification of new populations of chicken natural killer (NK) cells.
    Vervelde L
    Developmental and comparative immunology 34.7 (2010 Jul): 759-67.

    IF References
    FACS References

    Properties of and proteins associated with the extracellular ATPase of chicken gizzard smooth muscle. A monoclonal antibody study.
    Kirley TL
    The Journal of biological chemistry 270.20 (1995 May 19): 11845-50.

    Identification of new populations of chicken natural killer (NK) cells.
    Vervelde L
    Developmental and comparative immunology 34.7 (2010 Jul): 759-67.

    Chicken TREM-B1, an Inhibitory Ig-Like Receptor Expressed on Chicken Thrombocytes.
    Viertlboeck BC
    PloS one 11.3 (2016): e0151513.

    All References

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