Antigen: lysosomal membrane glycoprotein (LEP-100) / CD107
Hybridoma Cells Available: Yes
Antigen Species: not certain
Depositor: Reichardt, L.
Host Species: mouse
Positive Tested Species Reactivity:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names:
Alternate Antibody Name: CD107
Alternate Antigen Name:
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID:
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5G10 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product 5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).