5G10

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$40.00
SKU: 5G10
View product citations for antibody 5G10 on CiteAb

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Available: 44

DSHB Data Sheet

Catalog Fields

Antigen: CD107/ LEP100/LAMP-1
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Reichardt, L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California San Francisco
Positive Tested Species Reactivity: Chick, Rat
Depositors Notes: The antigen is also known as lysosome-associated membrane glycoprotein 1 (LAMP-1) or lysosome membrane glycoprotein LEP100. This antibody clone is also known in the literature as ChCD107.
Antigen Molecular Weight: 95-105 kDa 48 kDa core protein
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMP1
Immunogen:
Alternate Gene Names: LEP100
Alternate Antibody Name: CD107
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P05300 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396220 
Additional Characterization:
Antibody Registry ID: AB_531769 
Additional Information: RRID:AB_531769
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5G10 was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product 5G10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • FACS References
  • All References
  • Initial Publication

    Developmental and comparative immunology
    Vervelde L
    Developmental and comparative immunology 34.7 (2010 Jul): 759-67.

    IF References

    Autophagy
    García-Del Portillo F
    Autophagy 12.10 (2016 Oct 2): 1886-1901.

    Autophagy
    Boya P
    Autophagy 5.7 (2009 Oct): 964-72.

    FACS References

    The Journal of biological chemistry
    Kirley TL
    The Journal of biological chemistry 270.20 (1995 May 19): 11845-50.

    Developmental and comparative immunology
    Vervelde L
    Developmental and comparative immunology 34.7 (2010 Jul): 759-67.

    PloS one
    Viertlboeck BC
    PloS one 11.3 (2016): e0151513.

    All References

    Autophagy
    García-Del Portillo F
    Autophagy 12.10 (2016 Oct 2): 1886-1901.

    Autophagy
    Boya P
    Autophagy 5.7 (2009 Oct): 964-72.

    The Journal of biological chemistry
    Kirley TL
    The Journal of biological chemistry 270.20 (1995 May 19): 11845-50.

    Developmental and comparative immunology
    Vervelde L
    Developmental and comparative immunology 34.7 (2010 Jul): 759-67.

    PloS one
    Viertlboeck BC
    PloS one 11.3 (2016): e0151513.

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