6-4H2

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$45.00 to $425.00
SKU: 6-4H2
View product citations for antibody 6-4H2 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: Myelin, CNS
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: De Blas, Angel L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: University of Connecticut
Antigen Molecular Weight:
Depositors Notes: This antibody reacts with myelin of the shiverer mouse, indicating that is not MBP or myelin proteolipid. Immunoblots with myelin show no reaction. The antigen might not be a protein or it might not react with denatured protein. The antibody reacts with paraformaldehyde perfused brain and oligodendrocytes in culture., but does not react with paraffin-embedded tissue.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2877129 
Additional Characterization:  
Recommended Applications: ELISA, Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6-4H2 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 6-4H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IHC References
  • ELISA References
  • All References
  • Initial Publication

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

    IHC References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    ELISA References

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

    All References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

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