6-4H2
Catalog Fields
Clone ID/Product Name: 6-4H2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2877129
Clonality: Monoclonal
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Antigen Name: Myelin, CNS
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/21/2018
Antigen Molecular Weight:
Depositor: De Blas, Angel L.
Antigen Sequence:
Depositor Institution: University of Connecticut
Antigen Species: Rat
Depositor Notes: This antibody reacts with myelin of the shiverer mouse, indicating that is not MBP or myelin proteolipid. Immunoblots with myelin show no reaction. The antigen might not be a protein or it might not react with denatured protein. The antibody reacts with paraformaldehyde perfused brain and oligodendrocytes in culture., but does not react with paraffin-embedded tissue.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6-4H2 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 6-4H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
3 References
Initial Publication
Mammalian brain antigens defined by monoclonal antibodies.
Cherwinski HM
Brain research 322.2 (1984 Nov 26): 277-87.
Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
de Blas AL
The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.