6-4H2
Catalog Fields
Antigen: Myelin, CNS
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: De Blas, Angel L.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: This antibody reacts with myelin of the shiverer mouse, indicating that is not MBP or myelin proteolipid. Immunoblots with myelin show no reaction. The antigen might not be a protein or it might not react with denatured protein. The antibody reacts with paraformaldehyde perfused brain and oligodendrocytes in culture., but does not react with paraffin-embedded tissue.
Predicted Species Reactivity:
Gene:
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Antibody Registry ID: AB_2877129
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6-4H2 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 6-4H2)
Storage and Handling Recommendations
Usage Recommendations
All cell products contain the antimicrobial ProClin. Click here for additional information.
3 References
Initial Publication
Mammalian brain antigens defined by monoclonal antibodies.
Cherwinski HM
Brain research 322.2 (1984 Nov 26): 277-87.
Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
de Blas AL
The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.