608TN-C

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$45.00 to $425.00
SKU: 608TN-C
View product citations for antibody 608TN-C on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Product Name/ID: 608TN-C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: TNC
Ab Isotype: RIgG
Gene Symbol:
Antibody Registry ID: AB_2941795 
Uniprot ID: P24821 
RRID:  
Entrez Gene ID: 3371 
Clonality: Monoclonal
Immunogen: Purified from embryonic fibroblast culture
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: X-Ag8-653
Epitope Mapped: Yes
Antigen Name: Tenascin-C, Fbn III domains
Epitope Location or Sequence: All fibronectin type III domain repeats
Alternate Antigen Name:
Deposit Date: 9/15/2022
Antigen Molecular Weight: 240.8 kDa
Depositor: Faissner, Andreas
Antigen Sequence:
Depositor Institution: Ruhr University, Bochum, Germany
Antigen Species: Human
Depositor Notes: Does not cross react with mouse tenascin-C. This antibody binds both glycosylated and non-gkycosylated TN-C isoforms. FFPE requires HIER using 10mM citrate buffer, pH 6.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: mAb 608TN-C binds to perivascular single cells in tissues and to the intercellular spaces in culture.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
608TN-C was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product 608TN-C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    FFPE References
    Epitope Map References
    All References

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