60BG1.3

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SKU: 60BG1.3-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 60BG1.3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_10573813 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Formalinized Porphyromonas gingivalis, strain W83
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: F0
Epitope Mapped: No
Antigen Name: Porphyromonas gingivalis hemagglutinin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/11/2010
Antigen Molecular Weight: Apparent: multiple bands between 40 and 65 kDa, and two bands around 14 kDa
Depositor: Gmuer, R.
Antigen Sequence:
Depositor Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Species: Porphyromonas gingivalis
Depositor Notes: This antibody is species specific. 60BG1.3 appears to detect the same set of bands than 61BG1.3 by western blot, suggesting that 60BG1.3 recognizes some or all of the agglutinins recognized by 61BG1.3.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Porphyromonas gingivalis
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
60BG1.3 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 60BG1.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • All References
  • Initial Publication

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    IF References
    WB References

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    ELISA References

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    All References

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