60BG1.3
Catalog Fields
Antigen: Porphyromonas gingivalis hemagglutinin
Hybridoma Cells Available: No
Antigen Species: Porphyromonas gingivalis
Depositor: Gmuer, R.
Isotype: MIgG2b
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Host Species: mouse
Depositors Notes: This antibody is species specific. 60BG1.3 appears to detect the same set of bands than 61BG1.3 by western blot, suggesting that 60BG1.3 recognizes some or all of the agglutinins recognized by 61BG1.3.
Positive Tested Species Reactivity: Porphyromonas gingivalis
Clonality: Monoclonal
Antigen Molecular Weight: Apparent: multiple bands between 40 and 65 kDa, and two bands around 14 kDa
Epitope Mapped: No
Immunogen: Formalinized Porphyromonas gingivalis, strain W83
Immunogen Sequence: Full length protein
Myeloma Strain: F0
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Antibody Registry ID: AB_10573813
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
60BG1.3 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 60BG1.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
Guggenheim B
Oral microbiology and immunology 3.4 (1988 Dec): 181-6.