60BG1.3

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$40.00
SKU: 60BG1.3-s
View product citations for antibody 60BG1.3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Porphyromonas gingivalis hemagglutinin
Hybridoma Cells Available: No
Antigen Species: Porphyromonas gingivalis
Depositor: Gmuer, R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Positive Tested Species Reactivity: Porphyromonas gingivalis
Depositors Notes: This antibody is species specific. 60BG1.3 appears to detect the same set of bands than 61BG1.3 by western blot, suggesting that 60BG1.3 recognizes some or all of the agglutinins recognized by 61BG1.3.
Antigen Molecular Weight: Apparent: multiple bands between 40 and 65 kDa, and two bands around 14 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Formalinized Porphyromonas gingivalis, strain W83
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: F0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10573813 
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
60BG1.3 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 60BG1.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • All References
  • Initial Publication

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    IF References
    WB References

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    ELISA References

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    All References

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