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SKU: 61BG1.3-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 61BG1.3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: rgpA/kgp/hagA
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10568665 
Uniprot ID: Q51838 Q51817 P59915 
Entrez Gene ID: 2552074 29256891 2551934 
Clonality: Monoclonal
Immunogen: Formalinized Porphyromonas gingivalis, strain W83
Immunogen Sequence: Full length protein
Myeloma Strain: F0
Epitope Mapped: Yes
Antigen Name: Gingipain/ P. gingivalis hemagglutinin
Epitope Location or Sequence: beta adhesin domain of protease RI, aa 907-331 of prepro-prpR1 [Uniprot: Q51838]
Alternate Antigen Name:
Deposit Date: 3/11/2010
Antigen Molecular Weight: Apparent: multiple bands between 31 and 65 kDa, two bands around 14 kDa, and higher MW bands at 113 and 180 kDa.
Depositor: Gmuer, R.
Antigen Sequence:
Depositor Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Species: Porphyromonas gingivalis
Depositor Notes: This antibody is species specific and recognizes the hemagglutinating proteases present on all tested strains of Porphyromonas gingivalis. The hemagglutinins recognized by 61BG1.3 include RgpA (Gingipain R1; also known as prpR1 or hemagglutinin HagE), Kgp (Lys-gingipain) and HagA (Hemagglutinin A).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Porphyromonas gingivalis
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
61BG1.3 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 61BG1.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    IF References
    WB References
    IHC References
    ELISA References
    FFPE References
    FB References
    Epitope Map References
    All References

    Comparative study of abdominal and thoracic aortic aneurysms: their pathogenesis and a gingival fibroblasts-based ex vivo treatment.
    Fournier BP
    SpringerPlus 4. (2015): 231.

    Validation of a quantitative real-time PCR assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model.
    Thurnheer T
    Journal of periodontal research 48.4 (2013 Aug): 517-26.

    Oral application of a periodontal pathogen impacts SerpinE1 expression and pancreatic islet architecture in prediabetes.
    Watanabe K
    Journal of periodontal research 52.6 (2017 Dec): 1032-1041.

    Production and characterization of monoclonal antibodies specific for Bacteroides gingivalis.
    Guggenheim B
    Oral microbiology and immunology 3.4 (1988 Dec): 181-6.

    Characterization of the Porphyromonas gingivalis antigen recognized by a monoclonal antibody which prevents colonization by the organism.
    Lehner T
    Journal of periodontal research 32.1 Pt 1 (1997 Jan): 54-60.

    The relationship between colonization and haemagglutination inhibiting and B cell epitopes of Porphyromonas gingivalis.
    Lehner T
    Clinical and experimental immunology 110.2 (1997 Nov): 285-91.

    Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. Construction of mutants with a combination of rgpA, rgpB, kgp, and hagA.
    Nakayama K
    The Journal of biological chemistry 274.25 (1999 Jun 18): 17955-60.

    Expression and immunogenicity of hemagglutinin A from Porphyromonas gingivalis in an avirulent Salmonella enterica serovar typhimurium vaccine strain.
    Progulske-Fox A
    Infection and immunity 68.2 (2000 Feb): 732-9.

    Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains.
    Nakayama K
    Microbiology (Reading, England) 148.Pt 4 (2002 Apr): 1183-1191.

    Passive immunization with monoclonal antibodies against Porphyromonas gingivalis in patients with periodontitis.
    Lehner T
    Infection and immunity 64.2 (1996 Feb): 422-7.

    The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis.
    Brady LJ
    Oral microbiology and immunology 16.3 (2001 Jun): 153-62.

    Expression of single-chain antibody against RgpA protease of Porphyromonas gingivalis in Lactobacillus.
    Hammarström L
    Journal of applied microbiology 100.2 (2006 Feb): 256-63.

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