62-3G1
Catalog Fields
Antigen: GABAA receptor/benzodiazepine receptor/Cl- channel complex
Hybridoma Cells Available: No
Antigen Species: Bovine
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Bovine, Chicken, Fish, Guinea Pig, Mouse, Rat, Salamander
Depositors Notes: This antibody recognizes both Beta 2 and Beta 3 subunits of GABA(A) receptors. 62-3G1 recognizes human protein in ICC(IF) only using PLP fixation. Does not work for IP or WB applications on human proteins.
Predicted Species Reactivity:
Gene: GABRB2/GABRB3
Immunogen: Affinity purified BZDR/GABAR from bovine cerebral cortex
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence: Extracellular N-terminus common to Beta2 and Beta 3 subunits; QSVNDPGNMSLVKET
Uniprot ID: P0C2W5
Immunogen Sequence: Full length protein
Entrez Gene ID: 540657
Antibody Registry ID: AB_2753216
Additional Information: The DNA sequences of the heavy light chains are available. See PUBMED ID: 31125364 .
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
62-3G1 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 62-3G1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
20 References
Initial Publication
Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
Friedrich P
The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.
Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
de Blas AL
The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.