62-3G1

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$40.00
SKU: 62-3G1-s
View product citations for antibody 62-3G1 on CiteAb

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Available: 18

DSHB Data Sheet

Catalog Fields

Antigen: GABAA receptor/benzodiazepine receptor/Cl- channel complex
Hybridoma Cells Available:
Antigen Species: Bovine
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Bovine, Chicken, Fish, Guinea Pig, Mouse, Rat, Salamander
Depositors Notes: This antibody recognizes both Beta 2 and Beta 3 subunits of GABA(A) receptors.
Antigen Molecular Weight: 57 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: GABRB2/GABRB3
Immunogen: Affinity purified BZDR/GABAR from bovine cerebral cortex
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence: Extracellular N-terminus common to Beta2 and Beta 3 subunits; QSVNDPGNMSLVKET
Uniprot ID: P0C2W5 
Immunogen Sequence: Full length protein
Entrez Gene ID: 540657 
Additional Characterization:
Antibody Registry ID:
Additional Information: The DNA sequences of the heavy light chains are available. See PUBMED ID: 31125364 .
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
62-3G1 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 62-3G1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

20 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.

    IF References
    WB References

    Glucose inhibition of glucagon secretion from rat alpha-cells is mediated by GABA released from neighboring beta-cells.
    Braun M
    Diabetes 53.4 (2004 Apr): 1038-45.

    Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.

    Coexistence of two beta subunit isoforms in the same gamma-aminobutyric acid type A receptor.
    De Blas AL
    The Journal of biological chemistry 272.26 (1997 Jun 27): 16564-9.

    Immunocytochemical localization of the beta(3) subunit of the gamma-aminobutyric acid(A) receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 413.4 (1999 Nov 1): 535-48.

    Immunocytochemical localization of the GABAA receptor in the rat brain.
    De Blas AL
    European journal of pharmacology 136.3 (1987 Apr 29): 451-3.

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    IHC References

    Bergmann glia GABA(A) receptors concentrate on the glial processes that wrap inhibitory synapses.
    De Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.24 (2002 Dec 15): 10720-30.

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas.
    de Blas AL
    The Journal of comparative neurology 280.1 (1989 Feb 1): 15-26.

    Expression of GABA and GABAA receptors by neurons of the subplate zone in developing primate occipital cortex: evidence for transient local circuits.
    Rakic P
    The Journal of comparative neurology 317.1 (1992 Mar 1): 91-101.

    Immunocytochemical localization of the beta(3) subunit of the gamma-aminobutyric acid(A) receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 413.4 (1999 Nov 1): 535-48.

    Aging-related subunit expression changes of the GABAA receptor in the rat hippocampus.
    De Blas AL
    Neuroscience 74.2 (1996 Sep): 341-8.

    Immunocytochemical localization of the GABAA receptor in the rat brain.
    De Blas AL
    European journal of pharmacology 136.3 (1987 Apr 29): 451-3.

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    Functional NMDA and GABAA receptors in pioneer neurons of the cortical marginal zone.
    Fairén A
    The European journal of neuroscience 11.9 (1999 Sep): 3351-4.

    Immunocytochemical localization of the GABAA/benzodiazepine receptor in the guinea pig cochlear nucleus: evidence for receptor localization heterogeneity.
    Altschuler RA
    Brain research 504.1 (1989 Dec 11): 173-9.

    Evidence for the participation of peripheral α5 subunit-containing GABAA receptors in GABAA agonists-induced nociception in rats.
    Rocha-González HI
    European journal of pharmacology 734. (2014 Jul 5): 91-7.

    IP References

    Coexistence of two beta subunit isoforms in the same gamma-aminobutyric acid type A receptor.
    De Blas AL
    The Journal of biological chemistry 272.26 (1997 Jun 27): 16564-9.

    Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.

    Immunocytochemical localization of the beta(3) subunit of the gamma-aminobutyric acid(A) receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 413.4 (1999 Nov 1): 535-48.

    Aging-related subunit expression changes of the GABAA receptor in the rat hippocampus.
    De Blas AL
    Neuroscience 74.2 (1996 Sep): 341-8.

    Molecular heterogeneity of the type I GABAA/benzodiazepine receptor complex.
    Vitorica J
    European journal of pharmacology 267.1 (1994 Mar 15): 123-8.

    Molecular characterization of type I GABAA receptor complex from rat cerebral cortex and hippocampus.
    Vitorica J
    Brain research. Molecular brain research 25.3-4 (1994 Sep): 225-33.

    Localization of GABAA receptor subtypes in the tiger salamander retina.
    Yazulla S
    Visual neuroscience 8.1 (1992 Jan): 57-64.

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    ELISA References

    Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.

    A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies.
    Seeburg PH
    Brain research 569.1 (1992 Jan 8): 57-62.

    Immunocytochemical localization of the beta(3) subunit of the gamma-aminobutyric acid(A) receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 413.4 (1999 Nov 1): 535-48.

    Epitope Map References
    All References

    Bergmann glia GABA(A) receptors concentrate on the glial processes that wrap inhibitory synapses.
    De Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.24 (2002 Dec 15): 10720-30.

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas.
    de Blas AL
    The Journal of comparative neurology 280.1 (1989 Feb 1): 15-26.

    Expression of GABA and GABAA receptors by neurons of the subplate zone in developing primate occipital cortex: evidence for transient local circuits.
    Rakic P
    The Journal of comparative neurology 317.1 (1992 Mar 1): 91-101.

    Immunocytochemical localization of the beta(3) subunit of the gamma-aminobutyric acid(A) receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 413.4 (1999 Nov 1): 535-48.

    Aging-related subunit expression changes of the GABAA receptor in the rat hippocampus.
    De Blas AL
    Neuroscience 74.2 (1996 Sep): 341-8.

    Immunocytochemical localization of the GABAA receptor in the rat brain.
    De Blas AL
    European journal of pharmacology 136.3 (1987 Apr 29): 451-3.

    Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric acidA receptor in the rat brain.
    De Blas AL
    The Journal of comparative neurology 350.2 (1994 Dec 8): 260-71.

    Functional NMDA and GABAA receptors in pioneer neurons of the cortical marginal zone.
    Fairén A
    The European journal of neuroscience 11.9 (1999 Sep): 3351-4.

    Immunocytochemical localization of the GABAA/benzodiazepine receptor in the guinea pig cochlear nucleus: evidence for receptor localization heterogeneity.
    Altschuler RA
    Brain research 504.1 (1989 Dec 11): 173-9.

    Evidence for the participation of peripheral α5 subunit-containing GABAA receptors in GABAA agonists-induced nociception in rats.
    Rocha-González HI
    European journal of pharmacology 734. (2014 Jul 5): 91-7.

    Glucose inhibition of glucagon secretion from rat alpha-cells is mediated by GABA released from neighboring beta-cells.
    Braun M
    Diabetes 53.4 (2004 Apr): 1038-45.

    A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies.
    Seeburg PH
    Brain research 569.1 (1992 Jan 8): 57-62.

    Differences in the retinal GABA system among control, spastic mutant and retinal degeneration mutant mice.
    Pinto LH
    Vision research 37.24 (1997 Dec): 3471-82.

    Monoclonal antibodies and conventional antisera to the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 615-22.

    Coexistence of two beta subunit isoforms in the same gamma-aminobutyric acid type A receptor.
    De Blas AL
    The Journal of biological chemistry 272.26 (1997 Jun 27): 16564-9.

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    Molecular heterogeneity of the type I GABAA/benzodiazepine receptor complex.
    Vitorica J
    European journal of pharmacology 267.1 (1994 Mar 15): 123-8.

    Molecular characterization of type I GABAA receptor complex from rat cerebral cortex and hippocampus.
    Vitorica J
    Brain research. Molecular brain research 25.3-4 (1994 Sep): 225-33.

    Localization of GABAA receptor subtypes in the tiger salamander retina.
    Yazulla S
    Visual neuroscience 8.1 (1992 Jan): 57-64.

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