65B12

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$40.00
SKU: 65B12
View product citations for antibody 65B12 on CiteAb

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Available: 8

DSHB Data Sheet

Catalog Fields

Antigen: TSLP
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Farr, A.G .
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: University of Washington
Positive Tested Species Reactivity: Mouse
Depositors Notes: Specificity note: active with mouse TSLP; other species not tested. Antigen characterization: functional inhibition of TSLP responsive cell line. Functional effects: does not block TSLP activity in vitro or in vivo; can be used with 28F12 for a TSLP ELISA assay.
Antigen Molecular Weight: about 15 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: partially purified murine thymus stromal lymphopoietin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63-Ag8.653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications:  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
65B12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 65B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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