Product Name/ID: 65B12
Available to For-Profits: Yes
Alternate Antibody Name:
Ab Isotype: RIgG2a
Antibody Registry ID: AB_2256510
Immunogen: partially purified murine thymus stromal lymphopoietin
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: No
Antigen Name: TSLP
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/20/2008
Antigen Molecular Weight: about 15 kDa
Depositor: Farr, A.G .
Depositor Institution: University of Washington
Antigen Species: mouse
Depositor Notes: Specificity note: active with mouse TSLP; other species not tested. Antigen characterization: functional inhibition of TSLP responsive cell line. Functional effects: does not block TSLP activity in vitro or in vivo; can be used with 28F12 for a TSLP ELISA assay.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
65B12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 65B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).