Available to For-Profits: Yes
Antigen Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Depositor: Farr, A.G .
Host Species: rat
Positive Tested Species Reactivity: Mouse
Depositors Institution: University of Washington
Antigen Molecular Weight: about 15 kDa
Depositors Notes: Specificity note: active with mouse TSLP; other species not tested. Antigen characterization: functional inhibition of TSLP responsive cell line. Functional effects: does not block TSLP activity in vitro or in vivo; can be used with 28F12 for a TSLP ELISA assay.
Immunogen: partially purified murine thymus stromal lymphopoietin
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: No
Antibody Registry ID: AB_2256510
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
65B12 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 65B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).