Antigen: Neuronal acetylcholine receptor subunit alpha-3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Mihovilovic, Mirta
Host Species: mouse
Depositors Institution: Duke University
Positive Tested Species Reactivity: Human, Rodent
Depositors Notes: This antibody recognizes thymic cells and stains a thymic cellular mesh.
Predicted Species Reactivity:
Immunogen: Recombinant extracellular domain with boost of cytoplasmic domain for the alpha-3 AcchR subunit
Alternate Gene Names: LNCR2, NACHRA3, PAOD2
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P32297
Immunogen Sequence: aminio acids 1-127 of the alpha-3 AcChR subunit as extracellular domian and 313 to 411 as cytoplasmic domain of the alpha-3 subunit
Entrez Gene ID: 1136
Antibody Registry ID: AB_2721952
Additional Information: This antibody was reported in an abstract for the annual Society for Neuroscience meeting. RRID:AB_2721952
Recommended Applications: ELISA, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6A12-11C was deposited to the DSHB by Mihovilovic, Mirta (DSHB Hybridoma Product 6A12-11C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).